In vitro propagation of Lonicera tolmatchevii (Caprifoliaceae), regressive relict endemic of the Sakhalin Island
UDC 582.973+58.085(571.642)
Abstract
The protocol for clonal micropropagation of the Sakhalin Island endemic Lonicera tolmatchevii Pojark. has been developed for the first time, including stages of introduction into culture, reproduction, rooting under in vitro conditions and acclimatization under ex vitro conditions. It was shown that explants obtained from three-month-old seedlings actively form axillary shoots on Murashige-Skoog medium with the addition of cytokinins (BAP, Kn) and no formation of adventitious shoots occurred. It was found that the most effective combination of growth regulators for micropropagation of Lonicera tolmatchevii is a combination of 2 mg/L BAP and 0.5 mg/L IBA which leads to the formation of an average of 9.9 new shoots with active growth. The use of BAP without auxins leads to a decrease of the multiplication factor. The use of NAA at a concentration of 1 mg/l together with cytokinins (BAP, Kn) only resulted in the formation of callus that did not have morphogenic activity. The resulting regenerated plants were rooted successfully on hormone-free Murashige-Skoog medium with half the macronutrients. It has been shown that using auxins (IBA, IAA) at a concentration of 1 mg/L complicates the rooting process through the formation of callus tissue at the base of the explant. Rooted plants can be easily acclimatized to ex vitro conditions using a mixture of peat and vermiculite in a 1 : 1 ratio.
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References
Cambecedes J., Duron M., Decourtye L. 1991. Adventitious bud regeneration from leaf explants of the shrubby ornamental honeysuckle, Lonicera nitida Wils. cv. ‘Maigrün’: effects of thidiazuron and 2,3,5-triiodobenzoic acid. Pl. Cell Rep. 10: 471–474. https://doi.org/10.1007/bf00233817
Dziedzic E. 2008. Propagation of blue honeysuckle (Lonicera caerulea var. kamtschatica Pojark.) in in vitro culture. J. Fruit Ornam. Pl. Res. 16: 93–100.
Фирсов Г. А. Жимолость Толмачёва – Lonicera tolmachevii Pojark. // Красная книга Российской Федерации. Растения и грибы. М.: ВНИИ “Экология”, 2024. С. 202.
Фирсов Г. А., Волчанская А. В., Ткаченко К. Г. Жимолость Толмачёва (Lonicera tolmatchevii Pojark., Caprifoliaceae) в Санкт-Петербурге // Hortus bot., 2017. Т. 12. С. 332–338. https://doi.org/10.15393/j4.art.2017.4663
Kadhim Z. K., Al-Shareefi M. H., Lateef S. M. 2019. Effect of growth regulators on in vitro micropropagation of blue honeysuckle (Lonicera caerulea L.) Res. Crops. 20(3): 635–641.
Karhu S. T. 1997a. Axillary shoot proliferation of blue honeysuckle. Pl. Cell Tissue Organ Cult. 48: 195–201. https://doi.org/10.1023/A:1005842022064
Karhu S. T. 1997b. Rooting of blue honeysuckle microshoots. Pl. Cell Tissue Organ Cult. 48: 153–159. https://doi.org/10.1023/A:1005768117246
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15(13): 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Недолужко В. А. Жимолость Толмачёва на Сахалине // Бюл. Глав. ботан. сада, 1983. Вып. 127. С. 29–34.
Ochatt S. J. 1991. Requirements for plant regeneration from protoplasts of the shrubby ornamental honeysuckle, Lonicera nitida cv. Maigrun. Pl. Cell Tissue Organ Cult. 25: 161–167. https://doi.org/10.1007/bf00042188
Orlova N. D., Molkanova O. I., Koroleva O. V. 2021. Improvement of clonal micropropagation technique of promising Lonicera caerulea L. cultivars. IOP Conf. Series: Earth and Environmental Science 941: 012030. https://doi.org/10.1088/1755-1315/941/1/012030
Osburn L. D., Yang X., Li Y., Cheng Z.-M. 2009. Micropropagation of japanese honeysuckle (Lonicera japonica) and amur honeysuckle (L. maackii) by shoot tip culture. J. Environ. Hort. 27(4): 195–199. https://doi.org/10.24266/0738-2898-27.4.195
Sedlak J., Paprstein F. 2008. In vitro propagation of blue honeysuckle. Hort. Sci. 34(4): 129–131. https://doi.org/10.17221/1871-HORTSCI
Шейко В. В. Результаты интродукции сахалинского эндемика Lonicera tolmatchevii Pojark. (Caprifoliaceae) // Бюл. Главн. ботан. сада, 2018. Т. 204, № 2. С. 21–28. https://doi.org/10.25791/BBGRAN.02.2018.037
Шейко В. В. Жимолость Толмачёва Lonicera tolmachevii Pojark. // Красная книга Сахалинской области: Растения и грибы. Кемерово: ООО «Технопринт», 2019. C. 60.
Шейко B. B., Таран A. A. Биология и распространение регрессивного сахалинского эндемика Lonicera tolmatchevii (Caprifoliaceae) // Бот. журн., 2017. Т. 102, № 12. С. 1657–1662. https://doi.org/10.1134/S0006813617120067
Trang N. T., Luan N. T., Hang P. T. L. 2016. Study on in vitro propagation of japanese honeysuckle (Lonicera japonica Thunb.) via the callus method. JS: NST 32(1S): 384–390. URL: https://js.vnu.edu.vn/NST/article/view/3573/3163
Трусов Н. А., Яценко И. О., Рысин С. Л., Сорокопудов В. Н., Александров Д. С. Коллекция рода Lonicera L. в дендрарии ГБС РАН: история, современное состояние и перспективы развития // Современное садоводство, 2023. № 2. С. 20–50. https://doi.org/10.52415/23126701_2023_0203
Wang X., Chen J., Li Y., Nie Qi., Li J. 2009. An efficient procedure for regeneration from leaf-derived calluses of Lonicera macranthoides ‘Jincuilei’, an important medicinal plant. Hortscience 44(3): 746–750. https://doi.org/10.21273/HORTSCI.44.3.746
Zapolsky Ya. S., Medvedeva T. V., Natalchuk T. A., Bublyk M. O. 2018. Propagation of edible honeysuckle (Lonicera edulis Turch.) in in vitro conditions. Agric. Sci. Pract. 5(2): 18–26. https://doi.org/10.15407/agrip5.02.018
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