Стандарты в проточной цитометрии растений: обзор, вопросы полиморфизма и линейности

УДК 58.08+575.113+575.224.234.2

Ключевые слова: йодид пропидия, плоидность, проточная цитометрия растений, размер генома, содержание ДНК, стандартизация, эталонные стандарты, C-value

Аннотация

В статье представлен краткий обзор основных видов растений, используемых в качестве стандартов в проточной цитометрии. Проведена оценка их линейности на основе прямых измерений в течение года в условиях теплицы и открытого грунта. Значения содержания ДНК измерены в двух общепринятых в настоящее время значениях – на основе геномов человека (7,0 пг) и риса (0,795 пг). Выявлено, что среди значений содержания ДНК стандартов на основе генома человека, не линейны значения содержания ДНК у Raphanussativus и Solanum lycopersicum. В числе стандартов на основе генома риса выявлены отклонения в линейности для размеров геномов Pisum sativum, Vicia faba и Allium cepa. В работе впервые приведены наши прямые измерения содержания ДНК на основе размера генома риса для часто используемых стандартов, таких как ячмень, рожь, кукуруза и многих других. Выявлено, что нелинейность в основном связана со значением размера генома первоначального стандарта, инструментальными вариациями и полиморфизмом содержания ДНК стандартов. С помощью множественных стандартов исследованы некоторые виды многолетних растений, которые потенциально возможно использовать в качестве стандарта ввиду низкого полиморфизма содержания ДНК и удобства использования. В статье подробно исследованы особенности использования стандартов, а также акцентированно внимание на некоторых методических аспектах, обеспечивающих точность данных в проточной цитометрии растений.

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Опубликован
2024-08-01
Как цитировать
Скапцов М. В., Куцев М. Г., Смирнов С. В., Ваганов А. В., Уварова О. В., Шмаков А. И. Стандарты в проточной цитометрии растений: обзор, вопросы полиморфизма и линейности // Turczaninowia, 2024. Т. 27, № 2. С. 86-104 DOI: 10.14258/turczaninowia.27.2.10. URL: http://turczaninowia.asu.ru/article/view/15742.
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