Универсальные ПЦР-праймеры для идентификации видов рода Sanguisorba и других представителей семейства Rosaceae

УДК 582.734.4+575.174.015.3

  • Анастасия Максимовна Колтунова Алтайский государственный университет https://orcid.org/0000-0002-3753-7958 Email: koltunova.anas@yandex.ru
  • Максим Геннадьевич Куцев Алтайский государственный университет https://orcid.org/0000-0003-2284-6851 Email: m_kucev@mail.ru
  • Рамазан Алибегович Муртазалиев Прикаспийский институт биологических ресурсов Дагестанского ФИЦ РАН https://orcid.org/0000-0002-2895-213X Email: murtazaliev.ra@yandex.ru
Ключевые слова: видоспецифичные праймеры, кровохлебка, пластидный геном, филогенетический анализ

Аннотация

Разработано семь пар праймеров для амплификации некодирующих участков ДНК хлоропластов с помощью полимеразной цепной реакции (ПЦР). Для выяснения универсальности праймеров мы использовали их для амплификации фрагментов ДНК различных видов растений семейства Rosaceae. Праймеры являются пригодными для генотипирования видов рода Sanguisorba и некоторых видов семейства Rosaceae. С помощью данных праймеров возможно амплифицировать некодирующие полиморфные области хлоропластной ДНК, а значит, они могут быть использованы при выявлении межвидовых различий и в популяционной генетике растений.

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Литература

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Опубликован
2023-11-01
Как цитировать
Колтунова А. М., Куцев М. Г., Муртазалиев Р. А. Универсальные ПЦР-праймеры для идентификации видов рода Sanguisorba и других представителей семейства Rosaceae // Turczaninowia, 2023. Т. 26, № 3. С. 126-136 DOI: 10.14258/turczaninowia.26.3.10. URL: http://turczaninowia.asu.ru/article/view/14026.
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