Polymorphism of Cruciata krylovii coenopopulations in the Southern Siberia mountains based on morphological data and RAF-PCR analysis
Abstract
16 coenopopulations of the tertiary nemoral relic Cruciata krylovii (Rubiaceae) were studied in the forests of Western Sayan, Altai mountains and in the countryside of Tomsk city. C. krylovii coenopopulations expand at the fir and aspen deforested areas during the first few years. Reducing of projective coverage and parameters of vegetative organs of C. krylovii together with increasing of intrapopulation variability of morphological features were revealed. The most unfavourable areas for C. krylovii are fully deforested areas. Cluster analysis found that the coenopopulations from anthropogenically disturbed communities had morphological similarity. Genetic variability of the C. krylovii cenopopulations was studied by RAF-PCR method. The total percentage of polymorphic loci is 98.44. The level of intrapopulation genetic polymorphism varies within fairly wide limits (68.8-96.9 %). A high level of genetic variability is typical for coenopopulations growing in the chern, forest-steppe and subtaiga communities. All studied coenopopulations of the C. krylovii are characterized by an average level of genetic differentiation (Gst = 0.18) and are grouped according to the geographical principle. C. krylovii is revealed to be a phytocoenotical patient and explerent. High rates of morphological and genetic diversity of this species were revealed for the habitats from the border of the range (forest-steppe, subtaiga, Tomsk populations). It indicates the possibilities for the further settlement across the territory of Siberia. It has relatively wide ecological amplitude and resistant to anthropological pressure. However, deforested areas are not favor the spread of the species due to high competition from other plants. Following A. A. Grossgeim relict classification C. krylovii belongs to “adaptants”.
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