Optimization of in vitro micropropagation for Magnolia × soulangeana Soul.-Bod.
Abstract
This study was aimed to develop an efficiently repetitive protocol for micropropagation of Magnolia × soulangeana using shoot meristem. The effective scheme of bud surface sterilization was found. In our opinion the best sterilizing agent was thimerosal in concentration of 0,1 %. After growing in vitro cultures on different combinations of hormones, mediums supplemented with BAP, IAA, NAA, IBA were found to be most efficient and productive combination for shoot proliferation. MS medium with half salt composition showed itself as the most effective composition for shoot initiation in vitro. The most adequate for shoot multiplication was QL medium with adding BAP (1 mg/l). The proliferated shoots were transferred to different root induction media, which resultantly showed that WPM media supplemented with NAA (0.5 mg/l) was the most efficient root inducing media. Rooted plants were adapted to the green house conditions. For these purposes we used substrate consisted of turf, soil and sand in equal parts. Finally, these plants were successfully established in soil.Downloads
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