The karyotype of some rare species of Astragalus and Oxytropis (Fabaceae)
Abstract
The paper presents the results of a study of the karyotype of rare endemics: Astragalus chorinensis Bunge, Oxytropis glandulosa Turcz., O. stukovii Palibin, O. varlakovii Serg. On the basis of the data ideograms of chromosomes and karyotypes formulas are presented. The study showed that A. chorinensis somatic chromosome number was 2n = 16. The chromosomal formula is 2n = 2m + 14sm. The average of total length of the haploid chromosomes is 40.24 mm. The size of chromosomes varies from 4.29 ± 0.04 to 6.31 ± 0.06 microns. A. chorinensis is characterized by asymmetric karyotype with a predominance submetacentric chromosomes. Diploid set of chromosomes in O. glandulosa is 2n = 16. Chromosome formula is 2n = 16 = 4m + 12sm. Chromosomes are fairly uniform in size, the average length ranged from 1.56 ± 0.05 to 1.93 ± 0.09 m. The total length of the haploid chromosomes was 33.63 microns. For O. stukovii set chromosomal formula is 2n = 16 = 6m + 10sm. The total length of the haploid chromosomes (CL) was 33.74 microns. Chromosomes length ranges from 3.75 ± 0.10 m to 4.93 ± 0.14 m. O. varlakovii chromosomes are metacentric and submetacentric types. The chromosomal formula of 2n = 32 = 4m + 28sm. The size of chromosomes ranged from 0.74 ± 0.01 до 1.56 ± 0.15 microns. The total length of the haploid was 33.74 microns. O. stukovii, O. glandulosa and O. varlakovii are characterized by symmetrical karyotype with a predominance of metacentric chromosomes type.
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Abdel Samad F., Baumel A., Juin M., Pavon D., Siljak-Yakovlev S., Medial F., BouDagher Kharrat M. 2014. Phylogenetic diversity and genome sizes of Astragalus (Fabaceae) in the Lebanon biogeographical crossroad. Plant. Syst. Evol. 300: 819–830.
Arano H., Saito H. 1980. Cytological studies in family Umbelliferae 5. Karyotypes of seven species in subtribe Seselinae. Chromosoma 2, 17: 471–480.
Arslan E., Ertugrul K., Tugay O., Dural H. 2012. Karyological studies of the genus Onobrychis Mill. and the related genera Hedysarum L. and Sartoria Boiss. & Helder. (Fabaceae, Hedysareae) from Turkey. Cytologia 65(1): 11–17.
Arslanova L. R., Kalashnik N. A. 2009. Karyology of South Ural species of Oxytropis DC. Vestnik Orenburgskogo gosudarstvennogo universiteta [Vestnik of the Orenburg State University] 6: 43–45 [In Russian]. (Арсланова Л. Р., Калашник Н. А. Кариология южноуральских видов рода Oxytropis DC. // Вестник ОГУ, 2009. № 6. С. 43–45).
Badr A., Sharawy S. M. 2007. Karyotype analysis and systematic relationships in the Egyptian Astragalus L. (Fabaceae). International Journal of Botany 3: 147–159.
Baziz K., Benamara-Bellagha M., Pustahija F., Brown C. S., Siljak-Yakovlev S., Khalfallah N. 2014. First karyotype analysis, physical rDNA mapping and genome size assessment in four North African Astragalus taxa (Fabaceae). Turk. J. Bot. 38: 1248–1258.
Bennett M. D., Leitch I. J. 2005. Genome size in plants. In: The evolution of the genome. Ed. Gregory. Elsevier, San Diego, 162 pp.
Chepinoga V. V. 2014. Chromosome numbers of plant species from Baikal Siberia. Nauka, Novosibirsk, 419 pp. [In Russian]. (Чепинога В. В. Хромосомные числа растений флоры Байкальской Сибири. Новосибирск, 2014. 419 с.).
Chisla khromosom cvetkovykh rasteniy flory SSSR. T. 1. Semeystva Aceraceae – Menyanthaceae. [The number of chromosomes of flowering plants in the flora of the USSR. Vol. 1. Family Aceraceae – Menyanthaceae]. 1990. Nauka, Leningrad, 568 pp. [In Russian]. (Числа хромосом цветковых растений флоры СССР. Т. 1. Семейства Aceraceae – Menyanthaceae. Л.: Наука, 1990. 568 с.).
Dulepova B. I. 2002. Oxytropis stukovii Palibin. In: Krasnaya kniga Сhitinskoy oblasti i Aginskogo Buryatskogo avtonomnogo okruga. Rasteniya [The Red Book of the Chita Region and the Agin-Buryat Autonomous District. Plants]. Stil, Chita, 107 p. [In Russian]. (Дулепова Б. И. Остролодочник Стукова // Красная книга Читинской области и Агинского Бурятского автономного округа. Растения. Чита: Стиль, 2002. С. 107).
Filipov E. G., Kulikov P. V., Knjazev M. S. 2008. The numbers of chromosomes of species of Astragalus and Hedysarum (Fabaceae) the flora of Russia. Bot. Zhurn. (Moscow & St. Petersburg) 10: 1614–1620 [In Russian]. (Филиппов Е. Г., Куликов П. В., Князев М. С. Числа хромосом видов Astragalus и Hedysarum (Fabaceae) флоры России // Бот. журн., 2008. № 10. С. 1614–1620).
Grif V. G., Agapova N. D. 1986. To method of describing plant karyotypes. Bot. Zhurn. (Moscow & Leningrad) 71(4): 550–553 [In Russian]. (Гриф В. Г., Агапова Н. Д. К методике описания кариотипов растений // Бот. журн., 1986. Т. 71. № 4. С. 550–553).
Gubanov I. A. 1996. Konspekt flory Vneshnej Mongolii (sosudistye rasteniya) [Synopsis of the flora of Outer Mongolia (vascular plants)]. Valang, Moscow, 136 pp. [In Russian]. (Губанов И. А. Конспект флоры Внешней Монголии (сосудистые растения). М.: Валанг, 1996. 136 с.).
Kazem Y., Houshmand S., Zamani Dadane G. 2010. Karyotype analysis of Astragalus effusus Bunge (Fabaceae). Caryologia 63: 257–261.
Konichenko E. S., Selyutina I. Yu. 2013. Chromosome numbers of rare and endemic species of the genus Oxytropis (Fabaceae). Bot. Zhurn. (Moscow & St. Petersburg) 5: 647–651 [In Russian]. (Кониченко Е. С., Селютина И. Ю. Числа хромосом редких и эндемичных видов рода Oxytropis (Fabaceae) // Бот. журн., 2013. № 5. C. 647–651).
Konichenko E. S., Selyutina I. Yu., Dorogina O. V., Sandanov D. V. 2014. Karyotype studies endemic plant species Astragalus sericeocanus Gontsch. (Fabaceae) around Lake Baikal, Siberia. Caryologia 67(2): 172–177.
Krasnikov A. A. 2004. Methods of preparation of press time preparations for counting the chromosomes of plants. In: Problemy kariologii, karosistematiki i molekulyarnoy sistematiki rasteniy [Karyotype problems, Karyosystematics and molecular systematics of plants]. Novosibirsk, 10 pp. [In Russian]. (Красников А. А. Методика приготовления временных давленых препаратов для подсчета хромосом растений // Проблемы кариологии, кариосистематики и молекулярной систематики растений. Новосибирск, 2004. 10 с.).
Krivobokov L. V. 2013. Astragalus chorinensis Bunge. In: Krasnaya kniga Respubliki Buryatiya: rasteniya i griby [The Red Book of the Republic of Buryatia: Plants and mushrooms]. BSC SB RAS Publisher, Ulan-Ude, 515–516 pp. [In Russian]. (Кривобоков Л. В. Астрагал хоринский // Красная книга Республики Бурятия: Растения и грибы. Улан-Удэ: Изд-во БНЦ СО РАН, 2013. C. 515–516).
Malyshev L. I., Peshkova G. A. 1984. Osobennosti i genezis flory Sibiri [Features and genesis of Siberian flora]. Nauka, Novosibirsk, 264 pp. [In Russian]. (Малышев Л. И., Пешкова Г. А. Особенности и генезис флоры Сибири. Новосибирск: Наука, 1984. 264 с.).
Manandhar L., Sakya S. R. 2004. Cytotaxonomic studies in two species of Astragalus. J. Cytol. Genet. 5: 13–20.
Martin E., Duran A., Dinc M., Erisen S., Babaoglu M. 2008. Karyotipe Analyses of four Astragalus L. (Fabaceae) species from Turkey. Phytologia 90: 133–146.
Martin E., Erkul S. K. Aytae Z. 2015. Karyological studies Oxytropis (Fabaceae) from Turkey. Caryologia 68: 357–362.
Paszko B. 2006. A critical review and a new proposal of karyotype asymmetry indices. Plant. Syst. Evol. 258: 39–48.
Peruzzi L., Eroglu H. 2013. Karyotype asymmetry: again, how to measure and what to measure? Comparative cytogenetics 7(1): 1–9.
Peshkova G. A. 1972. Tertiary relicts in the steppe flora of the Baikal Siberia. In: Nauchnye chteniya pamyati M. G. Popova. 12–13 chteniya. [Scientific readings in memory of M. G. Popov. 12–13 reading]. Irkutsk, 25–58 pp. [In Russian]. (Пешкова Г. А. Третичные реликты в степной флоре Байкальской Сибири // Научные чтения памяти М. Г. Попова. 12–13 чтения. Иркутск, 1972. С. 25–58).
Peshkova G. A. 1979. Family Fabaceae or Leguminosae – Legumes. In: Flora Tsentralnoy Sibiri. T. 2. [Flora of the Central Siberia. Vol. 2]. Nauka, Novosibirsk, 585–639 pp. [In Russian]. (Пешкова Г. А. Семейство Fabaceae или Leguminosae – Бобовые // Флора Центральной Сибири Т. 2. Новосибирск: Наука, 1979. С. 585–639).
Peshkova G. A. 2008. Oxytropis glandulosa Turcz. In: Krasnaya kniga Rossiyskoy Federacii rasteniya i griby [The Red Book of the Russian Federation, plants and fungi]. Tovarishhestvo nauchnykh izdaniy, Moscow, 253 p. [In Russian]. (Пешкова Г. А. Остролодочник железистый // Красная книга Российской Федерации. Растения и грибы. М.: Товарищество научных изданий КМК, 2008. С. 253).
Ranjbar M., Karamian R., Bayat S. 2010. New chromosome counts in five species of Oxytropis (Fabaceae) in Iran. Iranian Journal of Botany 16 (1): 42–48.
Ranjbar M., Hadidchi A., Riahi H. 2014. Chromosome number reports in Astragalus sect. Onobrychoidei (Fabaceae) from Iran. Taxonomy and Biosystematics 21: 71–81.
Romero-Zarco C. 1986. A new method for estimating karyotype asymmetry. Taxon 35: 526–530.
Sandanov D. V., Chimitov D. G. 2013. Oxytropis glandulosa Turcz. In: Krasnaya kniga Respubliki Buryatiya: rasteniya i griby [The Red Book of the Republic of Buryatia: plants and mushrooms]. BSC SB RAS Publisher, Ulan-Ude, 515–516 pp. [In Russian]. (Санданов Д. В., Чимитов Д. Г. Остролодочник железистый // Красная книга Республики Бурятия: Растения и грибы. Улан-Удэ, Изд-во БНЦ СО РАН, 2013. C. 526).
Selyutina I. Yu., Sandanov D. V. 2015. Oxytropis stukovii Palib. – the rare species of Oxitropis DC. of the Estern Zabaikailye. Vestnik Buryatskogo gosudarstvennogo universiteta. Biologiya [Bulletin of Buryat State University] 4: 30–34 [In Russian]. (Селютина И. Ю., Санданов Д. В. Oxytropis stukovii Palib. – редкий вид остролодочника Восточного Забайкалья // Вестник БГУ, 2015. Т. 4. С. 30–34).
Ya-Hui L., Ying M., Yong-Hong Y., Yong-Ping Y. 2011. Chromosome number and karyotypes of six Oxytropis species (Fabaceae) from the Oinghai-Tibetan Plateau, China. Plant Diversity and Resource 33 (4): 423–431.
Zarubin A. M. 2010. Oxytropis varlakovii Serg. In: Krasnaya kniga Irkutskoy oblasti: Sosudistyye rasteniya [The Red Book of Irkutsk region: vascular plants]. Vremya stranstviy, Irkutsk, 257 p. [In Russian]. (Зарубин А. М. Остролодочник Варлакова // Красная книга Иркутской области: сосудистые растения. Иркутск: Время странствий, 2010. С. 257).
Zuo L., Yuan Q. 2011. The difference between the heterogeneity of the centromeric index and intrachromosomal asymmetry. Plant. Syst. Evol. 297: 141–145.
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