Karyotypes and nuclear DNA content in some Trollius L. and Hegemone Bunge ex Ledeb. (Ranunculaceae) species of Asian Russia and China
Abstract
The karyotypes of Trollius altaicus C. A. Mey., T. asiaticus L., T. ledebourii Rchb. и Hegemone lilacina Bunge have been studied. Somatic chromosome number 2n = 16 was determined for all the specimens investigated. Three Trollius species have chromosome complements similar to each other in length and morphological types of chromosomes (metacentric, submetacentric and subtelocentric ones). The karyotype formula of Trollius altaicus is 2n = 2x = 16 = 14sm + 2st, T. asiaticus is 2n = 2x = 16 = 2m + 10sm + 2sm/st + 2st, and T. ledebourii is 2n = 2x = 16 = 2m/sm + 10sm + 4st. We have studied the karyotype of Hegemone lilacina for the first time, and its formula is 2n = 2x = 16 = 4m + 8sm + 4st. Also, we have determined nuclear DNA content (C-value) for 8 species by the flow cytometry. Hegemone lilacina and Trollius yunnanensis Ulbr. have the highest C-values (9.80 ± 0.29 pg and 9.39 ± 0.29 pg respectively), while the lowest C-values belong to T. farreri Stapf (8.20 ± 0.24 pg) and H. micrantha (C. Winkl. et Komarov) Butkov (8.28 ± 0.25 pg). The nuclear DNA content of T. altaicus specimens found in different locations were identical (8.48 ± 0.25 and 8.51 ± 0.25 pg) and similar-sized with T. vicarius Sipliv. (8.45 ± 0.28 pg). Two T. asiaticus specimens found in different locations appeared to vary in C-value (8.66 ± 0.26 pg and 8.98 ± 0.28 pg), and to be similar-sized with T. chinensis Bunge (8.87 ± 0.26 pg).
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