In vitro propagation of rare species Rhodiola rosea from Altai Mountains
Keywords:
ex vitro acclimatization; in vitro propagation; planting material; Rhodiola rosea
Abstract
The techniques of biotechnology are considered promising and highly-demanded for propagation and conservation of rare species of medicinal plants. The variety of natural forms of Rhodiola rosea makes it difficult to develop a single efficient in vitro protocol for this valuable and rare medicinal plant. The optimization of in vitro methods for specific populations to effectively mass production of planting material R. rosea is required. The paper provides the technique for in vitro propagation of R. rosea from the population of Altai Mountains, including sterilization of seeds in an aqueous solution of sodium hypochlorite, planting of seedlings on Murashige and Skoog medium (MS) supplemented with 5 μM 6-benzylaminopurine (BAP) and 2.5 μM α-naphthylacetic acid (NAA), rooting of microshoots on hormone-free ½ MS medium and acclimatization in the substrate consisting of peat and perlite at a ratio of 2 : 1. We have shown that no additional pre-sowing treatment is required for effective in vitro germination of R. rosea seeds. The seedlings of R. rosea exhibit a high germination rate and are a promising explant type for in vitro mass propagation of the plant. This technique provides a high yield of in vitro cultivated plants (6.3 plants per explant, 95–100 % rooting) and a high percentage of plant acclimatization (78 %). The average weight of roots (wet weight) during acclimatization and growth (7 months) was 12.2 ± 2.4 g. The developed technique of in vitro propagation of R. rosea allows both to conserve the natural populations of this rare plant and to obtain planting material for commercialcultivation.
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References
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Dimitrov B., Tasheva K., Zagorska N., Evstatieva L. 2003. In vitro cultivation of Rhodiola rosea L. Genetics and Breeding 32(1‒2): 3‒6.
Furmanova M., Hartwich M., Alferman A. W. 2002. Glucosylation of p-tyrosol to salidroside by Rhodiola rosea L. cell cultures. Herba Pol. 48: 71‒76.
Furmanova M., Oledzka H., Michalska M., Sokolnicka I., Radomska D. 1995. XXII. Rhodiola rosea L. (Roseroot): in vitro Regeneration and the Biological Activity of Roots. In: Biotechnology in Agriculture and Forestry, Medicinal and Aromatic Plants. VIII. Ed. Y. P. S. Bajaj. Springer, Heidelberg 33: 412‒426.
Galambosi B. 2005. Rhodiola rosea L. from wild collection to field production. Medicinal Plant Conservation 11(1): 31‒35.
Grech-Baran M., Sykłowska-Baranek K., Pietrosiuk A. 2015. Biotechnological approaches to enhance salidroside, rosin and its derivatives production in selected Rhodiola spp. in vitro cultures. Phytochem. Rev. 14: 657–674.
György Z. 2006. Glycoside production by in vitro Rhodiola rosea cultures. Ph. D Thesis, University of Oulu, Oulu, Finland.
Hai-jun L., Bin G., Qiong Y., Yu-jun L., Chun-Zhao L. 2006. Tissue culture of four Rhodiola species. Acta Botanica Boreali-Occidentalia Sinica 26(10): 2023‒2027.
Ishmuratova M. M. 1998. Clonal micropropagation of Rhodiola rosea L. and R. iremelica Boriss. in vitro. Rastitelnyye resursy [Plant Resources] 34: 12‒23 [In Russian]. (Ишмуратова М. М. Клональное микроразмножение Rhodiola rosea L. и R. iremelica Boriss. in vitro // Растительные ресурсы, 1998. Т. 34. С. 12‒34).
Kaftanat V. N., Bodrug M. V., Floryia V. N. 1988. Enhanced multiplication of Rhodioloa rosea in Moldova. In: Proceedings of the Second National Conference on Medicinal Botany. Kiev, USSR, 64 p.
Khan M. Y., Aliabbas S., Kumar V., Rajkumar S. 2009. Resent Advances in Medcinal Plant Biotehology. Indian Journal of Biotechnology 8: 9‒22.
Kim E. F. 1999. Rodiola rozovaya (zolotoy koren) sem. Tolstyankovykh i biologicheskiye osnovy vvedeniya eye v kulturu [Rhodiola rosea (golden root) of the family Crassulaceae and biological basis of its introduction into the culture cultivation: abstract of the thesis of doctor of biological sciences]. Novosibirsk, 20 pp. [In Russian]. (Ким Е. Ф. Родиола розовая (золотой корень) сем. Толстянковых и биологические основы введения ее в культуру: автореф. … д-ра биол. наук. Новосибирск, 1999. 20 c.).
Kirichenko E. B., Rudenko S. S., Baglaj B. M., Masikevich U. G. 1994. Leaf culture from in vitro propagated Rhodiola rosea. Bulletin GBS, RAN 169: 50‒54.
Krajewska-Patan A., Dreger M.., Lowicka A.., Gorska-Paukszta M.., Mścisz A.., Mielcarek S., et al. 2007. Chemical investigation of biotransformed Rhodiola rosea callus tissue. Herba Pol. 53: 77‒87.
Kurkin V. A., Zapesochnaya G. G. 1986. Chemical composition and pharmacological properties of plants of the genus Rhodiola plants (review). Khimiko-farmatsevticheskiy zhurnal [Pharmaceutical Chemistry Journal] 20(10): 1231‒1244 [In Russian]. (Куркин В. А., Запесочная Г. Г. Химический состав и фармакологические свойства растений рода родиола (обзор) // Хим.-фармац. журн., 1986. Т. 20, № 10. С. 1231‒1244).
Lütken H., Meropi-Antypa N., Kemp O., Hegelund J. N., Müller R. 2017. Hairy root cultures of Rhodiola rosea to increase valuable bioactive compounds. In: Production of plant derived natural compounds through hairy root culture. Ed. S. Malik. Springer, Cham, 89‒116 pp. DOI: https://doi.org/10.1007/978-3-319-69769-7_4
Marchev A. S., Dinkova-Kostova A. T., György Z. 2016. Rhodiola rosea L.: from golden root to green cell factories. Phytochem. Rev. 15: 515–536. DOI: 10.1007/s11101-016-9453-5
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. plant 15(13): 473‒497.
Nekratova N. A. Rhodiola rosea L. 2008. In: Red Data Book of the Russian Federation (plants and fungi). Eds. R. V. Kamelin, V. S. Novikov. Partnership of scientific publications KMK, Moscow, 855 pp. [In Russian]. (Некратова Н. А. Rhodiola rosea L. // Красная книга Российской Федерации (растения и грибы). M.: Товарищество научных изданий КМК, 2008. 855 с.).
Ohba H. 2005. Rhodiola. Illustrated handbook of succulent plants: Crassulaceae. Berlin, 210‒227 pp.
Panossian A. Wikmana G. Sarris J. 2010. Rosenroot (Rhodiola rosea): Traditional use, chemical composition, pharmacology and clinical efficacy. Phytomedicine 17: 481–493. DOI: 10.1016/j.phymed.2010.02.002
Platikanov S., Evstatieva L. 2008. Introduction of wild golden root (Rhodiola rosea L.) as a potential economic crop in Bulgaria. Econ Bot. 62(4):621‒627.
Poletaeva I. I., Volodina S. O., Volodin V. V. 2013. Studying the individual variability of Rhodiola rosea L. plants for selection the valuable genotypes for microclonal reproduction. Izvestia of RAS Samara Scientific Center 15(3): 769‒775 [In Russian]. (Полетаева И. И., Володина С. О., Володин В. В. Изучение индивидуальной изменчивости растений Rhodiola rosea L. в целях отбора ценных генотипов для микроклонального размножения // Известия Самарского научного центра Российской академии наук. 2013. Т. 15, № 3(2). С. 769‒775).
Ramachandra Rao S., Ravishankar G. A. 2002. Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances 20(2): 101‒153.
Rhodiola rosea. Traditional herbal medicines for modern times. 2015. Eds. Alain Cuerrier, Kwesi Ampong-Nyarko, CRC Press, 282 pp.
Saratikov A. S., Krasnov E. A. 2004. Rodiola rozovaya (zolotoy koren) [Rhodiola rosea (golden root)]. Тоmsк, 292 pp. [In Russian]. (Саратиков А. С., Краснов Е. А. Родиола розовая (золотой корень). Томск, 2004. 292 с.).
Stepanova E. F., Shirzad B., Evseeva S. B. 2016. Rhodiola rosea: the state of the studies and the possibilities of creating cosmeceutical and dermatological medicinal product. Pharmacia and pharmacology 4 (5): 36‒62 [In Russian]. (Степанова Э. Ф., Ширзад Б., Евсеева С. Б. Родиола розовая: состояние исследований и возможности создания космецевтических и дерматологических средств // Фармация и фармакология, 2016. Т. 4, № 5. С. 36‒62). DOI: 10.19163/2307-9266-2016-4-5-36-62
Tasheva K., Kosturkova G. 2010. Bulgarian golden root in vitro cultures for micropropagation and reintroduction. Central European Journal of Biology 5(6): 853‒863.
Tasheva K., Kosturkova G. 2012. The role of biotechnology for conservation and biologically active substances production of Rhodiola rosea: Endangered Medicinal Species. The Scientific World Journal ID 274942. DOI: 10.1100/2012/274942
Verpoorte R., Contin A., Memelink J. 2002. Biotechnology for the production of plant secondary metabolites. Phytochemistry Reviews 1(1): 13‒25. DOI: 10.1023/A:1015871916833
Yin W. B., Li W., Du G. S., Huang Q. N. 2004. Studies on tissue culture of Tibetan Rhodiola rosea. Acta Bot. Boreal. Occident. Sin. 24: 1506‒1510.
Zakhozhyy I. G. 2006. Fiziologo-biokhimicheskiye osnovy nakopleniya produktov vtorichnogo metabolizma - salidrozida i rozavina v rasteniyakh Rhodiola rosea L. [Physiological and biochemical basis of accumulation of products of secondary metabolism - salidroside and rosavin in plants of Rhodiola rosea L.: abstract of the thesis of candidate of biological sciences]. St. Petersburg, 19 pp. [In Russian]. (Захожий И. Г. Физиолого-биохимические основы накопления продуктов вторичного метаболизма ‒ салидрозида и розавина в растениях Rhodiola rosea L. Автореф. … канд. биол. наук. СПб., 2006. 19 с.).
Dimitrov B., Tasheva K., Zagorska N., Evstatieva L. 2003. In vitro cultivation of Rhodiola rosea L. Genetics and Breeding 32(1‒2): 3‒6.
Furmanova M., Hartwich M., Alferman A. W. 2002. Glucosylation of p-tyrosol to salidroside by Rhodiola rosea L. cell cultures. Herba Pol. 48: 71‒76.
Furmanova M., Oledzka H., Michalska M., Sokolnicka I., Radomska D. 1995. XXII. Rhodiola rosea L. (Roseroot): in vitro Regeneration and the Biological Activity of Roots. In: Biotechnology in Agriculture and Forestry, Medicinal and Aromatic Plants. VIII. Ed. Y. P. S. Bajaj. Springer, Heidelberg 33: 412‒426.
Galambosi B. 2005. Rhodiola rosea L. from wild collection to field production. Medicinal Plant Conservation 11(1): 31‒35.
Grech-Baran M., Sykłowska-Baranek K., Pietrosiuk A. 2015. Biotechnological approaches to enhance salidroside, rosin and its derivatives production in selected Rhodiola spp. in vitro cultures. Phytochem. Rev. 14: 657–674.
György Z. 2006. Glycoside production by in vitro Rhodiola rosea cultures. Ph. D Thesis, University of Oulu, Oulu, Finland.
Hai-jun L., Bin G., Qiong Y., Yu-jun L., Chun-Zhao L. 2006. Tissue culture of four Rhodiola species. Acta Botanica Boreali-Occidentalia Sinica 26(10): 2023‒2027.
Ishmuratova M. M. 1998. Clonal micropropagation of Rhodiola rosea L. and R. iremelica Boriss. in vitro. Rastitelnyye resursy [Plant Resources] 34: 12‒23 [In Russian]. (Ишмуратова М. М. Клональное микроразмножение Rhodiola rosea L. и R. iremelica Boriss. in vitro // Растительные ресурсы, 1998. Т. 34. С. 12‒34).
Kaftanat V. N., Bodrug M. V., Floryia V. N. 1988. Enhanced multiplication of Rhodioloa rosea in Moldova. In: Proceedings of the Second National Conference on Medicinal Botany. Kiev, USSR, 64 p.
Khan M. Y., Aliabbas S., Kumar V., Rajkumar S. 2009. Resent Advances in Medcinal Plant Biotehology. Indian Journal of Biotechnology 8: 9‒22.
Kim E. F. 1999. Rodiola rozovaya (zolotoy koren) sem. Tolstyankovykh i biologicheskiye osnovy vvedeniya eye v kulturu [Rhodiola rosea (golden root) of the family Crassulaceae and biological basis of its introduction into the culture cultivation: abstract of the thesis of doctor of biological sciences]. Novosibirsk, 20 pp. [In Russian]. (Ким Е. Ф. Родиола розовая (золотой корень) сем. Толстянковых и биологические основы введения ее в культуру: автореф. … д-ра биол. наук. Новосибирск, 1999. 20 c.).
Kirichenko E. B., Rudenko S. S., Baglaj B. M., Masikevich U. G. 1994. Leaf culture from in vitro propagated Rhodiola rosea. Bulletin GBS, RAN 169: 50‒54.
Krajewska-Patan A., Dreger M.., Lowicka A.., Gorska-Paukszta M.., Mścisz A.., Mielcarek S., et al. 2007. Chemical investigation of biotransformed Rhodiola rosea callus tissue. Herba Pol. 53: 77‒87.
Kurkin V. A., Zapesochnaya G. G. 1986. Chemical composition and pharmacological properties of plants of the genus Rhodiola plants (review). Khimiko-farmatsevticheskiy zhurnal [Pharmaceutical Chemistry Journal] 20(10): 1231‒1244 [In Russian]. (Куркин В. А., Запесочная Г. Г. Химический состав и фармакологические свойства растений рода родиола (обзор) // Хим.-фармац. журн., 1986. Т. 20, № 10. С. 1231‒1244).
Lütken H., Meropi-Antypa N., Kemp O., Hegelund J. N., Müller R. 2017. Hairy root cultures of Rhodiola rosea to increase valuable bioactive compounds. In: Production of plant derived natural compounds through hairy root culture. Ed. S. Malik. Springer, Cham, 89‒116 pp. DOI: https://doi.org/10.1007/978-3-319-69769-7_4
Marchev A. S., Dinkova-Kostova A. T., György Z. 2016. Rhodiola rosea L.: from golden root to green cell factories. Phytochem. Rev. 15: 515–536. DOI: 10.1007/s11101-016-9453-5
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. plant 15(13): 473‒497.
Nekratova N. A. Rhodiola rosea L. 2008. In: Red Data Book of the Russian Federation (plants and fungi). Eds. R. V. Kamelin, V. S. Novikov. Partnership of scientific publications KMK, Moscow, 855 pp. [In Russian]. (Некратова Н. А. Rhodiola rosea L. // Красная книга Российской Федерации (растения и грибы). M.: Товарищество научных изданий КМК, 2008. 855 с.).
Ohba H. 2005. Rhodiola. Illustrated handbook of succulent plants: Crassulaceae. Berlin, 210‒227 pp.
Panossian A. Wikmana G. Sarris J. 2010. Rosenroot (Rhodiola rosea): Traditional use, chemical composition, pharmacology and clinical efficacy. Phytomedicine 17: 481–493. DOI: 10.1016/j.phymed.2010.02.002
Platikanov S., Evstatieva L. 2008. Introduction of wild golden root (Rhodiola rosea L.) as a potential economic crop in Bulgaria. Econ Bot. 62(4):621‒627.
Poletaeva I. I., Volodina S. O., Volodin V. V. 2013. Studying the individual variability of Rhodiola rosea L. plants for selection the valuable genotypes for microclonal reproduction. Izvestia of RAS Samara Scientific Center 15(3): 769‒775 [In Russian]. (Полетаева И. И., Володина С. О., Володин В. В. Изучение индивидуальной изменчивости растений Rhodiola rosea L. в целях отбора ценных генотипов для микроклонального размножения // Известия Самарского научного центра Российской академии наук. 2013. Т. 15, № 3(2). С. 769‒775).
Ramachandra Rao S., Ravishankar G. A. 2002. Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances 20(2): 101‒153.
Rhodiola rosea. Traditional herbal medicines for modern times. 2015. Eds. Alain Cuerrier, Kwesi Ampong-Nyarko, CRC Press, 282 pp.
Saratikov A. S., Krasnov E. A. 2004. Rodiola rozovaya (zolotoy koren) [Rhodiola rosea (golden root)]. Тоmsк, 292 pp. [In Russian]. (Саратиков А. С., Краснов Е. А. Родиола розовая (золотой корень). Томск, 2004. 292 с.).
Stepanova E. F., Shirzad B., Evseeva S. B. 2016. Rhodiola rosea: the state of the studies and the possibilities of creating cosmeceutical and dermatological medicinal product. Pharmacia and pharmacology 4 (5): 36‒62 [In Russian]. (Степанова Э. Ф., Ширзад Б., Евсеева С. Б. Родиола розовая: состояние исследований и возможности создания космецевтических и дерматологических средств // Фармация и фармакология, 2016. Т. 4, № 5. С. 36‒62). DOI: 10.19163/2307-9266-2016-4-5-36-62
Tasheva K., Kosturkova G. 2010. Bulgarian golden root in vitro cultures for micropropagation and reintroduction. Central European Journal of Biology 5(6): 853‒863.
Tasheva K., Kosturkova G. 2012. The role of biotechnology for conservation and biologically active substances production of Rhodiola rosea: Endangered Medicinal Species. The Scientific World Journal ID 274942. DOI: 10.1100/2012/274942
Verpoorte R., Contin A., Memelink J. 2002. Biotechnology for the production of plant secondary metabolites. Phytochemistry Reviews 1(1): 13‒25. DOI: 10.1023/A:1015871916833
Yin W. B., Li W., Du G. S., Huang Q. N. 2004. Studies on tissue culture of Tibetan Rhodiola rosea. Acta Bot. Boreal. Occident. Sin. 24: 1506‒1510.
Zakhozhyy I. G. 2006. Fiziologo-biokhimicheskiye osnovy nakopleniya produktov vtorichnogo metabolizma - salidrozida i rozavina v rasteniyakh Rhodiola rosea L. [Physiological and biochemical basis of accumulation of products of secondary metabolism - salidroside and rosavin in plants of Rhodiola rosea L.: abstract of the thesis of candidate of biological sciences]. St. Petersburg, 19 pp. [In Russian]. (Захожий И. Г. Физиолого-биохимические основы накопления продуктов вторичного метаболизма ‒ салидрозида и розавина в растениях Rhodiola rosea L. Автореф. … канд. биол. наук. СПб., 2006. 19 с.).
Published
2018-12-11
How to Cite
Erst A., Erst A., Shmakov A. In vitro propagation of rare species Rhodiola rosea from Altai Mountains // Turczaninowia, 2018. Vol. 21, № 4. P. 78-86 DOI: 10.14258/turczaninowia.21.4.9. URL: http://turczaninowia.asu.ru/article/view/4771.
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