DNA imaging cytometry in plant analysis: a review
UDC 58.086+576.08+57.086.88
Keywords:
FCM, flow cytometry, genome size, hybrids, ICM, image cytometry, plants, polyploids
Abstract
The review focuses on image cytometry of plant cells, used to determine the ploidy level and genome size of plants. The review presents examples of basic plant studies using the method of analyzing static images of nuclei, the dyes used, sample preparation methods, data analysis, laboratory equipment and software. It also provides an application of the image cytometry in the study of vascular plants. An important parameter of any method is reproducibility and comparative characteristics relative to other methods. The article compares absolute values of plant genome size measurements made by image cytometry and flow cytometry, as well as the necessary minimum parameters to ensure measurement accuracy and limitations of the method. The review will be useful when planning an experiment on DNA content analysis without using expensive equipment – flow cytometers, but only on the basis of optical or fluorescence microscopy data.
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Gregory T. R. 2003. Genome size estimates for two important freshwater molluscs, the zebra mussel (Dreissena polymorpha) and the schistosomiasis vector snail (Biomphalaria glabrata). Genome 46: 841–844.
Greilhuber J. 2008. Cytochemistry and C-values: the less-well-known world of nuclear DNA amounts. Ann. Bot. Fenn. 101: 791 804.
Greilhuber J., Doležel J., Lysák M., Bennett M.D. 2005. The origin, evolution, and proposed stabilization of the terms ‘genome size’ and ‘C-value’ to describe nuclear DNA contents. Ann. Bot. Fenn. 95: 255–260.
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Published
2024-11-10
How to Cite
Kutsev M. G., Skaptsov M. V., Koltunova A. M., Uvarova O. V. DNA imaging cytometry in plant analysis: a review // Turczaninowia, 2024. Vol. 27, № 3. P. 141–158 DOI: 10.14258/turczaninowia.27.3.14. URL: http://turczaninowia.asu.ru/article/view/16101.
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Science articles
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