Karyotype and genome size in Adonis vernalis and Adonis volgensis
Abstract
The comparative karyological analysis of Adonis vernalis and Adonis volgensis (Ranunculaceae) from 11 populations from Russia (Altai Territory, Novosibirsk Region, Republic of Khakassia) and Bulgaria was carried out. The somatic chromosome number was 2n = 16 in all studied plants. Chromosome sets of A. vernalis from the Altai Territory (near Sibirskiye Ogni village) and the Republic of Khakassia (Tashtipskiy District), A. volgensis from the Altai Territory (near Chernopyatovo village) and Bulgaria (Kavarna Municipality) consist of eight metacentric and eight submetacentric chromosomes. Two pairs of submetacentric chromosomes had small satellites in short arms. The karyotype formula of these plants was 2n = 2x = 16 = 8m + 4sm + 4smsat. The specimen with the chromosome rearrangement (nonreciprocal translocation) was found in the Altai Territory population. The chromosome mutation has changed the chromosome morphology of submetacentric pair to metacentric pair of different size. The karyotype formula of this specimen was 2n = 2x = 16 = 2n = 10m + 6sm. A similar mutation was previously found out in A. apennina (A. sibirica) from the Tomsk Region. The nuclear DNA content (2C-value) was determined in eight Adonis populations by flow cytometry. The average absolute nuclear DNA content in A. vernalis attained on from 18.88 ± 1.01 to 19.47 ± 1.17 pg, and in A. volgensis attained on from 16.10 ± 0.41 to 16.47 ± 0.47 pg. Differences in 2C-value between A. volgensis and A. vernalis correlated with differences in total chromosome lengths of the set.
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Бадаева Е. Д., Салина Е. А. Структура генома и хромосомный анализ растений // Вавиловский журнал генетики и селекции, 2013. Т. 17, № 4/2. С 1017–1043.
Cronquist A. 1981. An integrated system of classification of flowering plants. New York: Columbia University Press. 1262 pp.
Doležel J., Sgorbati S., Lucretti S. 1992. Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiologia Plantarum 85: 625–631. DOI: 10.1111/j.1399-3054.1992.tb04764.x
Горовой П. Г., Волкова С. А. Новый вид рода Adonis L. (Ranunculaceae) c ocтрова Сахалин // Turczaninowia, 2021. Т. 24, № 3. С. 85–88. DOI: 10.14258/turczaninowia.24.3.7
Kubešová M., Moravcová L., Suda J., Jarošík V., Pyšek P. 2010. Naturalized plants have smaller genomes than their non-invading relatives: a flow cytometric analysis of the Czech alien flora. Preslia 82: 81–96.
Kurita M. 1955. Cytological studies in Ranunculaceae IV. The karyotype analysis in Actaea and some other genera. Jap. Jour. Genet. 30: 124–127.
Levan A., Fredgam K., Sandberg A. 1964. Nomenclature for centrometric position of chromosomes. Hereditas 52: 201–220.
Луферов А. Н. Таксономический конспект лютиковых (Ranunculaceae) Дальнего Востока России // Turczaninowia, 2004. Т. 7, № 1. С. 5–84.
Луферов А. Н. Род Adonis L. – Адонис, Горицвет // Флора Нижнего Поволжья. Т. 2: Раздельнолепестные двудольные цветковые растения. Часть 1. Salicaceae – Droseraceae. Отв. ред. Н. М. Решетников; Главный ботанический сад им. Н. В. Цицина РАН. М.: Тов-во науч. изд. KMK, 2018. C. 347–350.
Луферов А. Н. К диагностике видов Adonis (Ranunculaceae) флоры России // Новейшие зарубежные и отечественные препараты, 2020. № 2. С. 3–14. DOI: 10.33920/med-06
Meusel H., Jaeger E., Weinert E. 1965. Vergleichende Chorologie der Zentraleuropӓischen Flora. Stuttgart: G. Fischer. 258 pp.
Mitrenina E. Yu., Erst A. S., Skaptsov M. V., Leonova T. V., Veklich T. N., Chernysheva O. A., Luferov A. N., Wang W. 2021. Karyotypes and genome size of Adonis amurensis and Adonis apennina (Ranunculaceae) from Asian Russia. Ukrainian Journal of Ecology 11(1): 163–170.
Nishikawa T. 1989. Botanical studies on Adonis amurensis Regel et Radde in Japan. Part 2. J. Hokkaido Univ. Educ., Sect. 2B, 39(2): 1–25.
Nishikawa T., Ito K. 1979. The chromosome numbers of Adonis amurensis Regel & Radde (sensu lato) of northern Honshu. J. Jap. Bot. 54(12): 353–362.
Nishikawa T., Kadota Y. 2006. Adonis. In: K. Iwatsuki K. et al. (eds). Flora of Japan. V. IIa. Tokyo: Kodansha Ltd. Pp. 287–288.
Parfitt B. D. 1997. Adonis L. In: Flora of North America Committee, eds. Flora of North America. Vol. 3. Magnoliophyta: Magnoliidae and Hamamelidae. Oxford: Oxford University Press. Pp. 184–187.
Paszko A. 2006. A critical review and a new proposal of karyotype asymmetry indices. Pl. Syst. Evol. 258: 39–48. DOI: 10.1007/s00606-005-0389-2
Peruzzi L., Eroğlu H. E. 2013. Karyotype asymmetry: Again, how to measure and what to measure? Comparative Cytogenetics 7: 1–9. DOI: 10.3897/compcytogen.v7i1.4431
Пошкурлат А. П. Род горицвет – Adonis L. Систематика, распространение, биология. М.: Наука, 2000. 199 с.
Ren Y., Chang H. L., Tian X. H., Song P., Endress P. K. 2009. Floral development in Adonideae (Ranunculaceae). Flora 204: 506–517.
Rice A., Glick L., Abadi Sh., Einhorn M., Kopelman N. M., Salman-Minkov A., Mayzel J., Chay O., Mayrose I. 2015. The Chromosome Counts Database (CCDB) – a community resource of plant chromosome numbers. New Phytol. 206(1): 19–26.
Ricroch A., Yockteng R., Brown S. C., Nadot S. 2005. Evolution of genome size across some cultivated Allium species. Genome 48: 511–520. DOI: 10.1139/g05-017
Шлангена З. Е. Хромосомные числа некоторых видов рода Adonis L. СССР (Ranunculaceae) // Бот. журн., 1976. Т. 61, № 11. С. 1603–1608.
Шрагер Л. Н. Полиморфизм спутничных хромосом нескольких популяций Adonis sibirica Patr. // Проблемы популяционной и эволюционной цитогенетики растений и животных. Томск: Изд-во Томского ун-та, 1980. С. 51–56.
Шрагер Л. Н., Малахова Л. А. Особенности кариотипа Adonis sibirica Patr. из Томской области // Цитология, 1978. Т. 20, № 5. С. 592–596.
Шрагер Л. Н., Малахова Л. А. Анализ кариотипов Adonis vernalis и Clematis integrifolia (Ranunculaceae) // Бот. журн., 1981. Т. 66, № 3. С. 438–440.
Smirnov S., Skaptsov M., Shmakov A., Fritsch R., Friesen N. 2017. Spontaneous hybridization among Allium tulipifolium and A. robustum (Allium subg. Melanocrommyum, Amaryllidaceae) under cultivation. Phytotaxa 303(2): 155–164. DOI: 11646/phytotaxa.303.2.5
Смирнов Ю. А. Ускоренный метод исследования соматических хромосом плодовых // Цитология, 1968. Т. 10, № 12. С. 1601–1602.
Son D. C., Park B. K., Ko S. C. 2016. Phylogenetic study of the section Adonanthe of genus Adonis L. (Ranunculaceae) based on ITS sequences. Korean Journal of Plant Taxonomy 46:1–12.
Tamura M. 1991. A new classification of the family Ranunculaceae 2. Acta Phytotaxonomica et Geobotanica 42: 177−187.
Волкова С. А., Горовой П. Г., Пшенникова Л. М. Кариотипы Adonis amurensis (Ranunculaceae) из Приморского края и Сахалина // Turczaninowia, 2020. Т. 23, № 2. С. 39–48. DOI: 10.14258/turczaninowia.23.2.6
Wang W. T. 1994a. Revision of Adonis (Ranunculaceae) (1). Bull. Bot. Research 14(1): 1–31.
Wang W. T. 1994b. Revision of Adonis (Ranunculaceae) (2). Bull. Bot. Research 14(2): 105–138.
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