Somaclonal variation of Elecampane, Inula britannica L. in culture in vitro
Abstract
The effect of somaclonal variation in callus culture and regenerants of elecampane – Inula britannica L. was studied. Calluses were cultured in medium in the presence of auxin and cytokinin for 12 months followed by stimulation of organogenesis. Polymorphism of calluses and regenerants were examined by fragment analysis using RAF-markers. Degree of genomic changes in callus cell cultures was assessed by flow cytometry. Analysis of the percentage of polymorphic loci demonstrated increasing genetic diversity on stages of callus cultures, whereas the induced polymorphism was restored to the original level at the regeneration phase. Changes in the size of regenerant’s genome were not readily identified in the first month of culturing, whereas in some samples the variations in genome of regenerants after 12 months callus culturing reached 26 %. Moreover, 12 month subcultured regenerants formed mutations in the generativeorgans of plants.
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