In vitro establishment and TDZ-induced regeneration from shoot and leaf explants of Rhododendron sichotense Pоjаrk.
Abstract
Rhododendron sichotense Pоjаrk. (Ericaceae) is a winter-hardy species native to the Russian Far East, highly valued as an attractive ornamental woody plant. In order to establish of in vitro culture without the problem with exudation of polyphenols and to induce multiplication from leave and shoot explants of field grown plants, the procedure including the pre-cultivation of explants on hormone free Andersоn Medium (AM0) for 3 days followed by cultivation on AM supplemented with varying thidiazuron (TDZ) concentrations (0.1, 0.5, 1.0 µM) was developed. The elongation on hormone-free AM0 allowed obtaining the healthy shoots without any anomalies. The best response in terms of the regeneration frequency (50 %), the shoot number per explant (41.60 ± 5.25) and the shoot length (12.20 ± 1.04 mm) was achieved under 0.5 µM of TDZ from leaf explants.
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Anderson W. C. 1984. A revised tissue culture medium for shoot multiplication of rhododendron. The Journal of the American Society for Horticultural Science 109: 343–347.
Dewir Y., Nurmansyah N., Teixeira da Silva J. 2018. Thidiazuron-induced abnormalities in plant tissue cultures. Plant Cell Rep. 37: 1451–1470. DOI: 10.1007/s00299-018-2326-1
Eeckhaut T., Janssens K., Keyser E., Riek J. 2010. Micropropagation of Rhododendron. In: Protocols for in vitro propagation of ornamental plants: Methods in molecular biology. Eds. S. Jain, S. Ochatt. New York: Humana Press. Pp. 141–152. DOI: 10.1007/978-1-60327-114-1_14
Jones M., Yi Z., Murch S., Saxena P. K. 2007. Thidiazuron-induced regeneration of Echinacea purpurea L.: micropropagation in solid and liquid culture systems. Plant Cell Rep. 26: 13–19. DOI: 10.1007/s00299-006-0209-3
Карпова Е. А., Каракулов А. В. Флавоноиды некоторых видов рода Rhododendron L. флоры Сибири и Дальнего Востока // Химия растительного сырья, 2013. № 2. С 119–126.
Murthy B., Murch S., Saxena P. 1998. Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cellular & Developmental Biology – Plant 34: 267–275. DOI: 10.1007/BF02822732
Novikova T. I., Asbaganov S. V., Ambros E. V., Zaytseva Y. G. 2020. TDZ-induced axillary shoot proliferation of Rhododendron mucronulatum Turcz. and assessment of clonal fidelity using DNA-based markers and flow cytometry. In Vitro Cellular & Developmental Biology – Plant 56: 307–317. DOI: 10.1007/s11627-019-10049-9
Novikova T. I., Zaytseva Y. G. 2018. TDZ-induced morphogenesis pathways in woody plant culture. In: Thidiazuron: From Urea Derivative to Plant Growth Regulator. Eds. N. Ahmad, M. Faisal. Singapore: Springer Nature. Pp. 61–94. DOI: 10.1007/978-981-10-8004-3_3
Uchendu E., Paliyath G., Brown D., Saxena P. 2011. In vitro propagation of North American ginseng (Panax quinquefolius p. L.). In Vitro Cellular & Developmental Biology – Plant 47: 710–718. DOI: 10.1007/s11627-011-9379-y
Васильева О. В. Биолого-морфологические основы клонального микроразмножения некоторых представителей рода Rhododendron L.: Автореф. дис. ... канд. биол. наук. М., 2009. 20 с.
Врищ Д. Л., Варченко Л. И., Урусов В. М. Род рододендрон (Rhododendron L.) на Сихотэ-Алине: география, экология, генезис, хозяйственные перспективы // Вестник КрасГАУ, 2010. Т. 10. С. 64–71.
Woo S., Wetzstein H. 2008. Morphological and histological evaluations of in vitro regeneration in Elliottia racemosa leaf explants induced on media with thidiazuron. The Journal of the American Society for Horticultural Science 133: 167–172.
Зайцева Ю. Г., Новикова Т. И. Размножение в культуре in vitro некоторых морозоустойчивых представителей рода Rhododendron // Растительный мир Азиатской России, 2017. № 4(28). С. 66–72. DOI: 10.21782?RMAR1995-2449-2017-4(66-72)
Zaytseva Y. G., Novikova T. I. 2018. Morpho-histological analysis of shoot regeneration and large-scale propagation of an endangered species Rhododendron mucronulatum Turcz. Sib. J. For. Sci. 4: 20–28. DOI: 10.15372/SJFS20180403
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