Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)
Abstract
In order to preserve the gene pool of medicinal plant Oxytropis lanata (Pall.) DC. we analyzed allozyme polymorphism and identified reliable and informative marker enzyme systems of this species; also we studied the response of seeds to deep freezing in liquid nitrogen (–196 ºС). Population has an average level of polymorphism (P95 = 41,2 %, P99 = 52,9 %, A = 1,58, Ho = 0,158, He = 0,171) in general typical for herbaceous legumes, and can serve as a source of material for gene pool conservation of the species. Deep freezing has not led to the death of the seeds; it was marked stimulatory effect of ultralow temperatures, expressed as an acceleration of germination and sharp increase of germinability (98,6 ± 2,3 %) compared to the control (12,0 ± 3,5 %) that is associated with overcoming physical dormancy. There were no abnormalities in the development of seedlings from seeds passed cryopreservation.Downloads
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Blinova K.F., Iriste V.A. The present state and the prospects of investigation of the representatives of the genus Oxytropis DC. // Rast. Resursy [Plant Resources], 1972. – Vol. 8, iss. 2. – P. 305–309 [in Russian]. (Блинова К.Ф., Иристе В.А. Современное состояние и перспективы изучения представителей рода Oxytropis DC. // Раст. ресурсы, 1972. – Т. 8, вып. 2. – С. 305–309).
Blinova K.F., Sakanyan E.I. Species of Oxytropis DC. used in Tibetan medicine and their flavonoid composition // Rast. Resursy [Plant Resources], 1986. – Vol. 22, iss. 2. – P. 266–272 [in Russian]. (Блинова К.Ф., Саканян Е.И. Виды Oxytropis DC., применяемые в тибетской медицине, и их флавоноидный состав // Раст. ресурсы, 1986. – Т. 22, вып. 2. – С. 266–272).
Boikov T.G., Sutkin A.V. Ecophytocenotic features of Vicia tsydenii Malysch. in Southern Transbaikalia // Rus. J. Ecol., 2012. – No. 5. – P. 340–346.
Engelmann F. Plant cryopreservation: progress and prospects // In Vitro Cellular and Developmental Biology – Plant, 2004. – Vol. 40, No. 5. – P. 427–433.
Enzyme Nomenclature. Recommendations (1972) of the International Union of Biochemistry on the nomenclature and classification of enzymes. – Moscow: VINITI, 1979. – 321 p. [in Russian]. (Номенклатура ферментов. Рекомендации (1972 г.) Международного биохимического союза по номенклатуре и классификации ферментов. – М.: ВИНИТИ, 1979. – 321 с.).
Goncharenko G.G., Padutov V.E., Potenko V.V. Rukovodstvo po issledovaniyu khvoinykh vidov metodom elektroforeticheskogo analiza izofermentov [Guide to conifer species research by isozyme electrophoretic methods]. – Gomel: Polespechat, 1989. – 164 p. [in Russian]. (Гончаренко Г.Г., Падутов В.Е., Потенко В.В. Руководство по исследованию хвойных видов методом электрофоретического анализа изоферментов. – Гомель: Полеспечать, 1989. – 164 с.).
Hamrick J.L., Godt M.J.W. Effects of life history traits on genetic diversity in plant species // Philos. Trans. R. Soc. Lond. B., 1996. – Vol. 351. – P. 1291–1298.
Kasyanova L.N., Azovskiy M.G. Vegetation of dune sands of Olkhon Island on Baikal and some questions concerning its protection // Geogr. Nat. Res., 2011. – Vol. 32, No. 3. – P. 248–253 [in Russian]. (Касьянова Л.Н., Азовский М.Г. Растительность дюнных песков острова Ольхон на Байкале и вопросы ее охраны // География и природные ресурсы, 2011. – Т. 32, № 3. – С. 57–63).
Kasyanova L.N., Azovskiy M.G., Mazukabzov A.M. The vegetation of drift sands on an island Olkhon (Baikal) // Bull. Mosc. Soc. Natur., 2007. – Vol. 211, iss. 2. – P. 41–49 [in Russian]. (Касьянова Л.Н., Азовский М.Г., Мазукабзов А.М. Структура растительности перевеиваемых песков острова Ольхон (озеро Байкал) // Бюлл. МОИП, 2007. – Т. 211, вып. 2. – С. 41–49).
Kholina A.B., Koren O.G., Zhuravlev Yu.N. Allozyme variation in Oxytropis retusa Matsum. from the Kuril Archipelago // Natural History Research. Special Issue, 2000. – No. 7. – P. 15–20.
Kholina A.B., Koren O.G., Zhuravlev Yu.N. High polymorphism and autotetraploid origin of the rare endemic species Oxytropis chankaensis Jurtz. (Fabaceae) inferred from allozyme data // Rus. J. Genet., 2004. – Vol. 40, No. 4. – P. 393–400.
Kholina A.B., Koren O.G., Zhuravlev Yu.N. Genetic structure and differentiation of populations of the tetraploid species Oxytropis chankaensis (Fabaceae) // Rus. J. Genet., 2009. – Vol. 45, No. 1. – P. 70–80.
Kholina A.B., Nakonechnaya O.V., Yakubov V.V., Koren O.G. Genetic variation in six species of the genus Oxytropis DC. (Fabaceae) from Kamchatka Peninsula // Rus. J. Genet., 2013. – Vol. 49, No. 10. – P. 1021–1029. doi: 10.1134/S1022795411120088.
Kholina A.B., Voronkova N.M. Conserving the gene pool of far eastern plants by means of seed cryopreservation // Biol. Bull., 2008. – Vol. 35, No. 3. – P. 262–269.
Kholina A.B., Voronkova N.M. Seed cryopreservation of some medicinal legumes // Journal of Botany, 2012. – Vol. 2012, Article ID 186891, 7 pages, doi:10.1155/2012/186891.
Konopleva E.V. Comparative characteristics of analgesic and antihypoxic effects of tinctures of some species Oxytropis DC. // Rast. resursy [Plant Resources], 1989. – Vol. 25, iss. 2. – P. 254–258 [in Russian]. (Коноплева Е.В. Сравнительная характеристика обезболивающего и противогипоксического действия настоев некоторых видов Oxytropis DC. // Раст. ресурсы, 1989. – Т. 25, вып. 2. – С. 254–258).
Li M.X., Lan Z.H., Wei L.L. et al. Phytochemical and biological studies of plants from the genus Oxytropis // Rec. Nat. Prod., 2012. – Vol. 6, No. 1. – P. 1–20.
Nikolaeva M.G., Razumova M.V., Gladkova V.N. Spravochnik po prorashchivaniyu pokoyashchikhsya semyan [Germination of Dormant Seeds: A Handbook]. – Leningrad: Nauka, 1985. – 348 p. [in Russian]. (Николаева М.Г., Разумова М.В., Гладкова В.Н. Справочник по проращиванию покоящихся семян. – Л.: Наука, 1985. – 348 с.).
Olennikov D.N., Rokhin A.V. Galactomannan of the locoweed (Oxytropis lanata (Pallas) DC) seeds // Appl. Bioch. Microbiol., 2010. –Vol. 46, No. 4. – P. 444–448.
Pence V.C. Cryopreservation of seeds of Ohio native plants and related species // Seed Sci. Technol., 1991. – Vol. 19. – P. 235–251.
Perez-Garsia F. Effect of cryopreservation, gibberellic acid and mechanical scarification on the seed germination of eight endemic species from the Canary Islands // Seed Sci. Technol., 2008. – Vol. 36. – P. 237–242.
Polozhij A.V. Oxytropis DC. // Flora Sibiri [Flora Sibiriae]. – Novosibirsk: Nauka, 1994. – Vol. 9. – P. 74–151 [in Russian]. (Положий А.В. Oxytropis DC. // Флора Сибири. T. 9. Fabaceae (Leguminosae). – Новосибирск: Наука, 1994. – T. 9. – С. 74–151).
Povydysh M.N., Bobyleva N.S., Bityukova N.V. Oxytropis DC. // Rastitelnye resursy Rossii: dikorastushchie tsvetkovye rasteniya, ikh komponentnyi sostav i biologicheskaya aktivnost. T. 3. Semeistva Fabaceae–Apiaceae [Plant resources of Russia: wild flowering plants, their component composition, and biological activity. Vol. 3. Families Fabaceae–Apiaceae] / Ed. Budantsev A.L. – St. Peterburg, Moscow: KMK, 2010. – P. 65–69 [in Russian]. (Повыдыш М.Н., Бобылева Н.С., Битюкова Н.В. Oxytropis DC. – Остролодочник // Растительные ресурсы России: дикорастущие цветковые растения, их компонентный состав и биологическая активность. Т. 3. Семейства Fabaceae–Apiaceae / Ред. Буданцев А.Л. – СПб., М.: КМК, 2010. – С. 65–69).
Probatova N.S., Kazanovsky S.G., Rudyka E.G. et al. Oxytropis lanata // In: Marhold K. (ed.), IAPT/IOPB chromosome data 12. Taxon, 2011. – Vol. 60, No. 6. – P. 1792, E53.
Rao N.K. Plant genetic resources: Advances conservation and use through biotechnology // African J. Biotechnol., 2004. – Vol. 3. – P. 136–145.
Salomăo A.N. Tropical seed species’ responses to liquid nitrogen exposure // Braz. J. Plant Physiol., 2002. – Vol. 14, No. 2. – P. 133–138.
Shibata T., Sakai E., Shimomura K. Effect of rapid freezing and thawing on hard-seed breaking in Astragalus mongolicus Bunge (Leguminosae) // J. Plant Physiol., 1995. – Vol. 147. – P. 127–131.
Stanwood P.C. Cryopreservation of seed germplasm for genetic conservation // Cryopreservation of plant cells and organs. – Boca Raton; Florida: CRC Press, Inc, 1985. – P. 200–226.
Tikhonova V.L. Long-term storage of seeds // Rus. J. Plant Phys., 1999. – Vol. 46, No. 3. – P. 400–408.
Voronkova N.M., Kholina A.B. An influence of temperature factor and scarification on seed germination and growth of seedlings of Sophora flavescens Soland. // Rast. Resursy [Plant Resources], 2003. – Vol. 39, iss. 1. – P. 43–49 [in Russian]. (Воронкова Н.М., Холина А.Б. Влияние температурного фактора и скарификации на прорастание семян и рост сеянцев Sophora flavescens Soland. // Раст. ресурсы, 2003. – Т. 39, вып. 1. – С. 43–49).
Voronkova N.M., Kholina A.B. Conservation of endemic species from the Russian Far East using seed cryopreservation // Biol. Bull., 2010. – Vol. 37, No. 5. – P. 496–501. doi: 10.1134/S1062359010050092
Voronkova N.M., Kholina A.B., Verkholat V.P. Plant biomorphology and seed germination in pioneer species of Kamchatka volcanoes // Biol. Bull., 2008. – Vol. 35, No. 6. – P. 599–605.
Yurtsev B.A. Conspectus of the section Baicalia Bge. of the genus Oxytropis DC. // Novosti Sist. Vyssh. Rast. [Novit. Syst. Pl. Vasc.], 1964. – Vol. 1. – P. 191–218 [in Russian]. (Юрцев Б.А. Конспект системы секции Baicalia Bge. рода Oxytropis DC. // Новости сист. высш. раст., 1964. – Т. 1. – С. 191–218).
Zhivotovskiy L.A. Populyatsionnaya biometriya [Population Biometry]. – Moscow: Nauka, 1991. –271 p. [in Russian]. (Животовский Л.А. Популяционная биометрия. – М.: Наука, 1991. – 271 с.).
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