Clonal micropropagation of Astragalus sericeocanus Gontsch. and content of phenolic compounds in vitro
Keywords:
composition and content of phenolic compounds; endemic species; micropropagation; organogenesis; seed germination
Abstract
In vitro propagation system for Astragalus sericeocanus Gontsch., an endemic species of the coast of Lake Baikal, was developed for the first time. This species is a source of valuable secondary metabolites with medicinal properties. The efficiency of mechanical and chemical scarifications for overcoming physical dormancy of seeds of this species was estimated. The highest percentage of seed germination (80.7 ± 0.88 %) at the minimum duration of germination (7.3 ± 0.33 days) was obtained by mechanical treatment. The effect of thidiazuron (TDZ; 0.05–2.0 mg/l) supplemented to B5 medium on regenerative potential of different explant types was studied. Efficient TDZ concentrations induced direct organogenesis from primary shoots of seedlings (regeneration frequency up to 100 %) and axillary buds of microshoots (up 31.1 %) were revealed. The results showed that 0.1 mg/l TDZ was the ptimal concentration for the development of axillary meristems of primary shoots. Following cultivation of microshoots on the medium for propagation supplemented with 0.2 mg/l TDZ resulted in formation of 11.4 ± 0.64 shoots per explant. Callus formation was observed on medium supplemented with 1.0 and 0.05 mg/l TDZ using hypocotyl and cotyledon explants. Analysis of secondary metabolites by high performance liquid chromatography (HPLC) showed that the composition and content of phenolic compounds in regenerants obtained on medium supplemented with 0.05–0.1 mg/l TDZ were comparable to in vivo plants, indicating high biosynthetic capacity of microplants. Quercetin, kaempferol, apigenin and izorhamnetin were identified in the hydrolysates of extracts of in vivo plant leaves. Hydrolysates of in vitro plants extracts were characterized by the absence of apigenin only.
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References
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Dewir Y. H., El-Sayed El-Mahrouk M., Naidoo Y. 2011. Effects of some mechanical and chemical treatments on seed germination of Sabal palmetto and Thrinax morrisii palms. AJCS 5(3): 248–253.
Erisen S., Atalay E., Yorgancilar M. 2011. The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. Turk. J. Bot. 35(5): 521–526. DOI:10.3906/bot-1009-74.
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Lin L.-Z., He X.-G., Lindenmaier M., Nolan G., Yang J., Cleary M., Qiu S.-X., Cordell A. G. 2000. Liquid chromatography-electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A. membranaceus. J. Chromatogr. A. 876(1–2): 87–95.
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Pathak M. R., Abido M. S. 2014. The role of biotechnology in the conservation of biodiversity. Journal of Experimental Biology and Agricultural Sciences 2(4): 352–363.
Ranal M. A., De Santana D. G. 2006. How and why to measure the germination process? Revista Brasil. Bot. 29(1): 1–11.
Rao S. R., Ravishankar G. A. 2002. Plant cell cultures: Chemical factories of secondary metabolites. Biotechnology Advances 20(2): 101–153. DOI: 10.1016/S0734-9750(02)00007-1.
Sandanov D. V., Krivenko D. A. 2013. Astragalus sericeocanus Gontsch. In: The Red Data Book of Republic of Buryatia: Rare and endangered species of animals, plants and fungi. Buryat Scientific Center SB RAS Publisher, Ulan-Ude, 518–519 pp. [In Russian]. (Санданов Д. В., Кривенко Д. А. Астрагал шелковисто-седой. Astragalus sericeocanus Gontsch. // Красная книга Республики Бурятия: Редкие и находящиеся под угрозой исчезновения виды животных, растений и грибов. Улан-Удэ: Изд-во БНЦ СО РАН, 2013. C. 518–519).
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Sy A., Grouzis M., Danthu P. 2001. Seed germination of seven Sahelian legume species. J. Arid. Environ. 49, 4: 875–882. DOI: 10.1006/jare.2001.0818.
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Yorgancilar M., Erisen S. 2011. The effect of thidiazuron (TDZ) on shoot regeneration of Astragalus schizopterus. Journal of Animal and Plant sciences 21(3): 519–524.
Zhmud E. V., Elisafenko T. V., Krivenko D. A., Verkhozina A. V., Zviagina N. S., Dorogina O. V. 2012. State of coenopopulations of Astragalus sericeocanus (Fabaceae), an endemic species of the eastern coast of Baikal lake. Bot. Zhurn. (Moscow & St. Peterburg) 97(10): 1310–1320 [In Russian]. (Жмудь Е. В., Елисафенко Т. В., Кривенко Д. А., Верхозина А. В., Звягина Н.С., Дорогина О. В. Состояние ценопопуляций Astragalus sericeocanus (Fabaceae) – эндемика восточного побережья озера Байкал // Бот. журн., 2012. Т. 97, № 10. С. 1310–1320).
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Basalma D., Uranbey S., Gurlek D., Ozcan S. 2008. TDZ-induced plant regeneration in Astragalus cicer. Afr. J. Biotechnol. 7(8): 955–959. DOI: 10.5897/AJB07.628.
Baskin J. M., Baskin C. C. 2004. A classification system for seed dormancy. Seed Sci. Res. 14(1): 1–16. DOI: https://doi.org/10.1079/SSR2003150.
Benson E. E., Danaher J. E., Pimbley I. M., Anderson C. T., Wake J. E., Daley S., Adams L. K. 2000. In vitro micropropagation of Primula scotica: a rare Scotish plant. Biodiversity & Conservation 9(6): 711–726. DOI: 10.1023/A:1008941726419.
Butenko R. G. 1999. Biologiya kletok vysshikh rasteniy in vitro i biotekhnologii na ikh osnove [Cell biology of higher plants in vitro and biotechnology on their base]. FBK-PRYESS, Moscow, 160 pp. [In Russian]. (Бутенко Р. Г. Биология клеток высших растений in vitro и биотехнологии на их основе. М.: ФБК-ПРЕСС, 1999. 160 с.).
Dewir Y. H., El-Sayed El-Mahrouk M., Naidoo Y. 2011. Effects of some mechanical and chemical treatments on seed germination of Sabal palmetto and Thrinax morrisii palms. AJCS 5(3): 248–253.
Erisen S., Atalay E., Yorgancilar M. 2011. The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. Turk. J. Bot. 35(5): 521–526. DOI:10.3906/bot-1009-74.
Gamborg O. L., Eveleigh D. E. 1968. Culture methods and detection of glucanases in suspension cultures of wheat and barley. Can. J. Biochem. 46(5): 417–421.
Global strategy for plant conservation: 2011-2020. 2012. Convention on biological diversity. BGGI, Richmond, UK, 36 pp.
Gorbunov Yu. N., Dzybov D. S., Kuzmin Z. E., Smirnov I. A. 2008. Methodological recommendations for botanic gardens on the reintroduction of rare and threatened plants. Grif & Co, Tula – Moscow, 53 pp. URL: http://www.mobot.org/bep/pdf/Reintroduction_manual_engl.pdf
Hare P. D., Staden J., Van Staden J. 1994. Inhibitory effect of TDZ on the activity of cytokinin oxidase isolated from soybean callus. Plant Cell Physiol. 35(8): 1121–1125.
Hussain Md. S., Fareed S., Ansari S., Rahman Md. A., Ahmad I. Z., Saeed M. 2012. Current approaches toward production of secondary plant metabolites. J. Pharm. Bioallied. Sci. 4(1): 10–20. DOI: 10.4103/0975-7406.92725.
Ionkova I. 1995. In vitro cultures and formation of secondary metabolites in Astragalus. In: Biotechnology in Agriculture and Foresty, Medicinal and Aromatic Plants, VIII. Ed. Y. P. Bajaj. Springer – Verlag, Berlin, 97–138 pp.
Ionkova I. 2009. Optimization of flavonoid production in cell cultures of Astragalus missouriensis Nutt. (Fabaceae). Phcog Mag. 5(18): 92–97.
International rules for seed testing (ISTA). 1993. Seed Science and Technology 21: 160–186.
Khramova E. P., Komarevtseva E. K. 2008. Variability of flavonoids composition in Potentilla fruticosa (Rosaceae) leaves at different age states in the conditions of the Mountain Altai. Plant Resources 44, 3: 96–102 [In Russian]. (Храмова Е. П., Комаревцева Е. К. Изменчивость флавоноидного состава листьев Potentilla fruticosa (Rosaceae) разных возрастных состояний в условиях Горного Алтая // Раст. ресурсы, 2008. Т. 44. № 3. С. 96–102).
Konichenko E. S., Selyutina I. Yu., Dorogina O. V., Sandanov D. V. 2014. Karyotype studies endemic plant species Astragalus sericeocanus Gontsch. (Fabaceae) around Lake Baikal, Siberia. Caryologia 67(2): 172–177. 10.1080/00087114.2014.931639.
Krivenko D. A. 2015. Endemiki pribaykalya Astragalus olchonensis Gontsch. i Astragalus sericeocanus Gontsch. (Fabaceae): ekologo-biologicheskie osobennosti tsenopopulyatsiy, voprosy filogenii, okhrana [Astragalus olchonensis Gontsch. and Astragalus sericeocanus Gontsch. (Fabaceae) are the endemics of Pribaikalye: ecological and biological characteristics of cenopopulations, phylogeny questions, concervation]. Abstract of Dis. ... PhD. biol. sciences. Tomsk, 18 pp. [In Russian]. (Кривенко Д. А. Эндемики Прибайкалья Astragalus olchonensis Gontsch. и Astragalus sericeocanus Gontsch. (Fabaceae): эколого-биологические особенности ценопопуляций, вопросы филогении, охрана: автореф. дис. … канд. биол. наук. Томск, 2015. 18 с.).
Lin L.-Z., He X.-G., Lindenmaier M., Nolan G., Yang J., Cleary M., Qiu S.-X., Cordell A. G. 2000. Liquid chromatography-electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A. membranaceus. J. Chromatogr. A. 876(1–2): 87–95.
Maksimov O. B., Kulesh N. I., Gorovoy P. G. 2002. Polifenoly dalnevostochnykh rasteniy [Polyphenols Far Eastern plants]. Dalnauka, Vladivostok, 332 pp. [In Russian]. (Максимов О. Б., Кулеш Н. И., Горовой П. Г. Полифенолы дальневосточных растений. Владивосток: Дальнаука, 2002. 332 с.).
Nikolaeva M. G. 2001. Ecological and physiological aspects of seed dormancy and germination (rewiew of investigations for the last century). Bot. Zhurn. (Moscow & St. Peterburg) 86(12): 1–14 [In Russian]. (Николаева М. Г. Эколого-физиологические особенности покоя и прорастания семян (итоги исследований за истекшее столетие) // Бот. журн, 2001. Т. 86, № 12. С. 1–14).
Novikova T. I. 2013. Use of biothechnological approaches for the conservation of plant biodiversity. Plant Life of Asian Russia 12(2): 119–128 [In Russian]. (Новикова Т. И. Использование биотехнологических подходов для сохранения биоразнообразия растений // Растительный мир Азиатской России, 2013. Т. 12, № 2. С. 119–128).
Pathak M. R., Abido M. S. 2014. The role of biotechnology in the conservation of biodiversity. Journal of Experimental Biology and Agricultural Sciences 2(4): 352–363.
Ranal M. A., De Santana D. G. 2006. How and why to measure the germination process? Revista Brasil. Bot. 29(1): 1–11.
Rao S. R., Ravishankar G. A. 2002. Plant cell cultures: Chemical factories of secondary metabolites. Biotechnology Advances 20(2): 101–153. DOI: 10.1016/S0734-9750(02)00007-1.
Sandanov D. V., Krivenko D. A. 2013. Astragalus sericeocanus Gontsch. In: The Red Data Book of Republic of Buryatia: Rare and endangered species of animals, plants and fungi. Buryat Scientific Center SB RAS Publisher, Ulan-Ude, 518–519 pp. [In Russian]. (Санданов Д. В., Кривенко Д. А. Астрагал шелковисто-седой. Astragalus sericeocanus Gontsch. // Красная книга Республики Бурятия: Редкие и находящиеся под угрозой исчезновения виды животных, растений и грибов. Улан-Удэ: Изд-во БНЦ СО РАН, 2013. C. 518–519).
Selyutina I. Yu., Konichenko E. S., Dorogina O. V. 2014. The possibility of using ISSR-markers for detection of genetic differentiation of populations of rare species of Astragalus sericeocanus (Fabaceae). Plant Life of Asian Russia 16, 4: 3–8 [In Russian]. (Селютина И. Ю, Кониченко Е. С., Дорогина О. В. Возможности использования ISSR-маркеров для выявления генетической дифференциации популяций редкого вида Astragalus sericeocanus (Fabaceae) // Растительный мир Азиатской России, 2014. Т. 16, № 4. С. 3–8).
Semenova G. P. 2007. Redkiye i ischezayushchiye vidy flory Sibiri [Rare and endangered species of Siberia flora]. Geo, Novosibirsk, 408 pp. [In Russian]. (Семенова Г. П. Редкие и исчезающие виды флоры Сибири. Новосибирск: Академ. изд-во «Гео», 2007. 408 с.).
Sidneva O. V. 2006. Khemotaksonomicheskoe issledovanie vidov sektsii Cenantrum Koch roda Astragalus L. (Fabaceae Lindl.) Sibiri [Chemotaxonomic study of Siberia Astragalus L species of the section Cenantrum Koch (Fabaceae Lindl.)]. Abstract of Dis. ... PhD. biol. sciences. Novosibirsk, 16 pp. [In Russian]. (Сиднева О. В. Хемотаксономическое исследование видов секции Cenantrum Koch рода Astragalus L. (Fabaceae Lindl.) Сибири: автореф. дис. … канд. биол. наук. Новосибирск, 2006. 16 с.).
Song Ch., Zheng Zh., Liu D., Hu Zh., Sheng W. 1997. Ptepocarpans and isoflavans from Аstragalus membranaceus (Fisch.) Bunge. Zhiwu Xuebao 39(12): 1169–1171.
Sy A., Grouzis M., Danthu P. 2001. Seed germination of seven Sahelian legume species. J. Arid. Environ. 49, 4: 875–882. DOI: 10.1006/jare.2001.0818.
Tang W., Eisenbrand G. 1992. Astragalus membranaceus (Fisch.) Bge. In: Chinese drugs of plant origin: chemistry, pharmacology and use in traditional and modern medicine. Springer – Verlag, Berlin, 191–197 pp.
Vydrina S. N. 1994. Astragalus L. In: Flora Sibiri [Flora of Siberia]. T. 9: Fabaceae (Leguminosae). Sibirskaya izdatelskaya firma VO "Nauka", Novosibirsk, 20–71 pp. [In Russian]. (Выдрина С. Н. Астрагал // Флора Сибири. Т. 9: Fabaceae (Leguminosae). Новосибирск: Сибирская издательская фирма ВО «Наука», 1994. С. 20–71).
Vysotskiy V. A. 1998. Biotekhnologicheskiye metody v sisteme proizvodstva ozdorovlennogo posadochnogo materiala i selektsii plodovykh i yagodnykh rasteniy [Biotechnological methods for production and breeding of redeveloped fruit and berry plant material]. Abstract of Dis. ... Dr. agricultural sciences. Moscow, 44 pp. [In Russian]. (Высоцкий В. А. Биотехнологические методы в системе производства оздоровленного посадочного материала и селекции плодовых и ягодных растений: автореф. дисс… д-ра с.-х. наук. М., 1998. 44 с.).
Yorgancilar M., Erisen S. 2011. The effect of thidiazuron (TDZ) on shoot regeneration of Astragalus schizopterus. Journal of Animal and Plant sciences 21(3): 519–524.
Zhmud E. V., Elisafenko T. V., Krivenko D. A., Verkhozina A. V., Zviagina N. S., Dorogina O. V. 2012. State of coenopopulations of Astragalus sericeocanus (Fabaceae), an endemic species of the eastern coast of Baikal lake. Bot. Zhurn. (Moscow & St. Peterburg) 97(10): 1310–1320 [In Russian]. (Жмудь Е. В., Елисафенко Т. В., Кривенко Д. А., Верхозина А. В., Звягина Н.С., Дорогина О. В. Состояние ценопопуляций Astragalus sericeocanus (Fabaceae) – эндемика восточного побережья озера Байкал // Бот. журн., 2012. Т. 97, № 10. С. 1310–1320).
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Published
2018-12-11
How to Cite
Ambros E., Kotsupy O., Novikova T., Vysochina G. Clonal micropropagation of Astragalus sericeocanus Gontsch. and content of phenolic compounds in vitro // Turczaninowia, 2018. Vol. 21, № 4. P. 87-99 DOI: 10.14258/turczaninowia.21.4.10. URL: http://turczaninowia.asu.ru/article/view/4772.
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Science articles
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