Preparation of an analytical standard for the absolute concentration method in pollen analysis using artificial intelligence
UDC 57.087.3+581.331.2
Abstract
To quantify the absolute concentration of pollen in geological sediments, the marker grain method is commonly used. Lycopodium spore tablets and aluminosilicate spheres are the two most commonly used markers. However, the production and acquisition of “Palynospheres” and Lycopodium tablets are associated with a number of difficulties. This work proposes a solution based on spores of Lycopodium sp., which are painted in bright colors, which allows them to be used even when working with those pollen spectra in which spores of the genus Lycopodium are naturally present. It is proposed to use AI to determine the concentration of spores in each analytical standard. Analytical standards suitable for use in establishing the absolute concentration of pollen in spore-pollen analysis have been obtained and are geographically accessible to palynologists from the Russian Federation.
Downloads
Metrics
References
Bonny A. P. 1972. A method for determining absolute pollen frequencies in lake sediments. New Phytol. 71: 393–405. https://doi.org/10.1111/j.1469-8137.1972.tb04086.x
Campbell J. F. E., Fletcher W. J., Hughes P. D. 2016. A comparison of pollen extraction methods confirms dense‐media separation as a reliable method of pollen preparation. J. Quat. Sci. 31(6): 631–640. https://doi.org/10.1002/jqs.2905
Davis M. B. 1966. Determination of absolute pollen frequency. Ecology 47(2): 310–311.
Faegri K., Iversen J. 1975. Textbook of pollen analysis. Copenhagen: Munksgaard. 237 pp.
Gardner G. D., Gray R. E. 1976. Tracing subsurface flow in karst regions using artificially colored spores. Bulletin of the Association of Engineering Geologists 13: 177–197.
Гричук В. П., Заклинская Е. Д. Анализ ископаемых пыльцы и спор и его применение в палеогеографии. М.: ОГИЗ, 1948. 217 с.
Jocher G., Chaurasia A., Qiu J. 2023. Ultralytics YOLO (Version 8.0.0). URL: https://github.com/ultralytics/ultralytics (Accessed 18 November 2024).
Kitaba I., Nakagawa T. 2017. Black ceramic spheres as marker grains for microfossil analyses, with improved chemical, physical, and optical properties. Quat. Int. 455: 166–169. https://doi.org/10.1016/j.quaint.2017.08.052
Nakagawa T., Hayashi R., Yokoyama Y. 1998. Dense‐media separation as a more efficient pollen extraction method for use with organic sediment/deposit samples: comparison with the conventional method. Boreas 27(1): 15–24. https://doi.org/10.1111/j.1502-3885.1998.tb00864.x
Pascal VOC Challenge [2007]. The PASCAL Visual Object Classes Challenge 2007 (VOC2007) Results. URL: http://host.robots.ox.ac.uk/pascal/VOC/voc2007/ (Accessed 18 November 2024).
Stockmarr J. 1971. Tablets with spores used in absolute pollen analysis. Pollen et Spores 13: 615–621.
Stockmarr J. 1973. Determination of spore concentration with an electronic particle counter. Danmarks Geol. Undersøgelse, Raekke 15: 87–89.
Turczaninowia is a golden publisher, as we allow self-archiving, but most importantly we are fully transparent about your rights.
Authors may present and discuss their findings ahead of publication: at biological or scientific conferences, on preprint servers, in public databases, and in blogs, wikis, tweets, and other informal communication channels.
Turczaninowia allows authors to deposit manuscripts (currently under review or those for intended submission to Turczaninowia) in non-commercial, pre-print servers such as ArXiv.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).