Is Lotus zhegulensis Klok. (Fabaceae) a separate species endemic of the Middle Volga Region or just a variety of a widespread L. corniculatus L.?

DOI_disc_logo https://doi.org/10.14258/turczaninowia.24.4.7
Keywords: Fabaceae, Lotus zhegulensis, population, population genetics, species, SSR markers, taxonomy

Abstract

We have studied several samples from Lotus corniculatus s. l. and L. zhegulensis populations with the use of SSR and sequenced nuclear (ITS) and plastid (atpB-rbcL, ndhC-trnV, rpl32-trnL(UAG), trnH-psbA, trnL-trnF) markers. We analysed 9 local populations from the Volga River valley ranging from Ulyanovsk in the north to Volgograd in the south, as well as two local populations from Moscow and Lipetsk Provinces. Analyses of SSR markers using the STRUCRURE program divided the sample into three genetic clusters. Populations from Moscow and Lipetsk Provinces, and three local populations from the ‘locus classicus’ location of L. zhegulensis in Samara Province appeared to be the most genetically differentiated while all the other populations were variously genetically admixed. Low pairwise Fst values indicate low genetic differentiation of Lotus populations and the intraspecific nature of the revealed diversity. The nuclear and plastid DNA sequences analyzed yielded little information. Substitutions and indels revealed were mostly autapomorphies characteristic of separate specimens or small groups of specimens but not of populations. Our study enables to firmly conclude that Lotus populations from the right bank of the Volga River in vicinity of Zhiguli upland described as L. zhegulensis are weakly genetically differentiated from other populations of Lotus corniculatus s. l. and, hence, cannot be regarded as a separate species.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Barykina R. P., Kramina T. E. 2006. A comparative morphological and anatomical study of the model legume Lotus japonicus and related species. Wulfenia 13: 33–56.
Clement M., Posada D., Crandall K. A. 2000. TCS: A computer program to estimate gene genealogies. Molec. Ecol. 9: 1657–1659. DOI: 10.1046/j.1365-294x.2000.01020.x
Doyle J. J., Doyle J. L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochemistry Bulletin, Botanical Society of America 19: 11–15.
Earl D. A., von Holdt B. M. 2012. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources 4: 359–361.
Evanno G., Regnaut S., Goudet J. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molec. Ecol. 14(8): 2611–2620.
Гомолко А. А. Морфологическая изменчивость видов комплекса Lotus corniculatus L. s. l. (Fabaceae) на территории Беларуси // Структура и динамика биоразнообразия: Сб. тез. конф. Минск: БГУ, 2019. С. 115–117.
Grant W. F. 1995. A chromosome atlas and interspecific-intergeneric index for Lotus and Tetragonolobus (Fabaceae). Canad. J. Bot. 73: 1787–1809.
Hall T. A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41: 95–98.
Hardy O. J., Vekemans X. 2002. SPAGeDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol. Ecol. Notes 2: 618–620. DOI: 10.1046/j.1471-8278.2002.00305.x
Ильина В. Н. Флора бобовых южных районов Самарской области // Фиторазнообразие Восточной Европы, 2008. № 5. С. 131–137.
Kai S., Tanaka H., Hashiguchi M., Iwata H., Akashi R. 2010. Analysis of genetic diversity and morphological traits of Japanese Lotus japonicus for establishment of a core collection. Breeding Science 60: 436–446.
Katoh K., Misawa K., Kuma K., Miyata T. 2002. MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 30: 3059–3066. DOI: 10.1093/nar/gkf436
Katoh K., Standley D. M. 2013. MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molec. Biol. Evol. 30: 772–780. DOI: 10.1093/molbev/mst010
Хлесткина Е. К., Чухина И. Г. Генетические ресурсы растений: стратегия сохранения и использования // Вестник Российской академии наук, 2020. Т. 90, № 6. С. 522–527. DOI: 10.31857/S0869587320060043
Клоков М. В. Заметка о некоторых критических видах лядвенца // Бот. мат. (Ленинград), 1953. Т. 15. С. 145–149.
Kopelman N. M., Mayzel J., Jakobsson M., Rosenberg N. A., Mayrose I. 2015. CLUMPAK: a program for identifying clustering modes and packaging population structure inferences across K. Molecular Ecology Resources 15(5): 1179–1191. DOI: 10.1111/1755-0998.12387
Kramina T. E. 1999. A contribution to the taxonomic revision of the Lotus corniculatus complex (Leguminosae, Loteae) in the European part of the former USSR. Syst. & Geogr. P1. 68: 265–279.
Kramina T. E., Meschersky I. G., Degtjareva G. V., Samigullin T. H., Belokon Y. S., Schanzer I. A. 2018. Genetic variation in the Lotus corniculatus complex (Fabaceae) in northern Eurasia as inferred from nuclear microsatellites and plastid trnL-trnF sequences. Bot. J. Linn. Soc. 188: 87–116. DOI: 10.1093/BOTLINNEAN/BOY042
Крамина Т. Е., Тихомиров В. Н. Внутрипопуляционная изменчивость отдельных морфологических признаков Lotus corniculatus L. s. l. (Leguminosae) на территории европейской части СССР // Бюл. МОИП. Отд. Биол., 1991. Т. 96, Вып. 6. С. 117–126.
Крупкина Л. И. Полевые исследования в Новгородской области с Тамарой Николаевной Онипко (Смекаловой) // Vavilovia, 2020. № 3, 1. С. 60–66. DOI: 10.30901/2658-3860-2020-1-60-66
Larsen K., Zertová A. 1963. On the variation pattern of Lotus corniculatus in Eastern Europe. Bot. Tidsskr. 59(3): 177–194.
Миняев Н. А., Улле З. Г. Лядвенец – Lotus L. // Флора Европейской части СССР. Т. 6. Л.: Наука, 1987. С. 103–115.
Плаксина Т. И. Самарская лука – феномен природы Среднего Поволжья // Вестник СамГУ, 1999. Т. 2, № 12. С. 158–171.
Pritchard J. K., Stephens M., Donnelly P. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945–959.
Саксонов С. В., Васюков В. М., Сенатор С. А., Ильина В. Н., Корчиков Е. С. Приложение. Список объектов растительного мира и лишайникообразующих грибов, исключенных из Красной книги Самарской области // Красная книга Самарской области. Т. 1. Самара: Изд-во Самарской государственной областной академии (Наяновой), 2017. С. 366–371.
Васюков В. М., Саксонов С. В., Сенатор С. А. Эндемичные растения бассейна Волги // Фиторазнообразие Восточной Европы, 2015. Т. 9, № 3. С. 27–43.
Published
2021-12-20
How to Cite
Kramina T. E., Meschersky I. G., Fedorova A. V., Vasilieva N. V., Stepanova N. Y., Schanzer I. A. Is Lotus zhegulensis Klok. (Fabaceae) a separate species endemic of the Middle Volga Region or just a variety of a widespread L. corniculatus L.? // Turczaninowia, 2021. Vol. 24, № 4. P. 73-83 DOI: 10.14258/turczaninowia.24.4.7. URL: http://turczaninowia.asu.ru/article/view/10773.
Issue
Vol 24 No 4 (2021): Turczaninowia
Section
Science articles

Turczaninowia is a golden publisher, as we allow self-archiving, but most importantly we are fully transparent about your rights.

Authors may present and discuss their findings ahead of publication: at biological or scientific conferences, on preprint servers, in public databases, and in blogs, wikis, tweets, and other informal communication channels.

Turczaninowia allows authors to deposit manuscripts (currently under review or those for intended submission to Turczaninowia) in non-commercial, pre-print servers such as ArXiv.

Authors who publish with this journal agree to the following terms:


    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).