Chromosome numbers in some alien plant species of Novosibirsk Region: post II

Chromosome numbers (2n) for 12 alien species (Apiaceae, Asteraceae, Brassicaceae, Campanulaceae, Fabaceae, Lamiaceae, Malvaceae, Onagraceae, Poaceae, Polygonaceae) from the Novosibirsk Region are given. For the first time for Russia, the number of chromosomes of Xanthogalum purpurascens (2n = 22) is given, for Siberia – Nepeta cataria (2n = 34), Malva pusilla (2n = 42) and Koenigia weyrichii subsp. weyrichii (2n = 20). For all studied species, short information on the general distribution and dispersal in the Novosibirsk Region, literature data on the number of chromosomes from the territory of Russia are presented.

Galeopsis ladanum and recently discovered and rare in the city of Novosibirsk: Senecio viscosus and Nepeta cataria. The chromosome numbers were determined by direct counting in metaphase on root meristem squash preparations. Seeds were germinated on sterile sand. The root tips were pretreated for two hours with 0.2 % colchicine water solution, fixed in 3 : 1 absolute ethanol glacial acetic acid and stained with 1 % acetic hematoxylin. Metaphase plates were observed under 100 × magnification by the Axioscope 40 (Karl Zeiss, Axio Lab) and photographed by the AxiCam MRc 5 digital camera.
For all species, the references on chromosome numbers revealed from the samples collected in Russia are given as well, as relevant information in the international database "The Chromosome Counts Database" (Rice et al., 2015) is reflected incompletely. Latin names of plants are given according to the "Catalog of Life" (Hassler, 2020). The herbarium samples will be transferred at the Herbarium of the Central Siberian Botanical Garden of Siberian Branch of the Russian Academy Sciences (NS, Novosibirsk).
Distribution: native to Caucasus, Turkey and Iran. Species is cultivated in botanical gardens, goes wild. It is preserved on the old fallows of the Central Siberian Botanical Garden, actively spreads in the nearby forests (Zykova, Shaulo, 2019).
The chromosome number was determined in Russia for the first time. The same chromosome number was reported for the Georgia (Vasilyeva et al., 1981;Vasilyeva, Pimenov, 1991), the Turkey (Pimenov et al., 1998).
Distribution: S. viscosus is native Western and Central Europe, and the Caucasus. The location of the species has been recorded since the 1980s in Siberia. For the Novosibirsk Region, it was recorded for the first time in 2019 (Ebel, Zykova, 2021).
The chromosome number is given for Western Siberia for the first time. The same chromosome number was determined for the Irkutsk Region (Chepinoga, 2014, and references therein) and the Primorye Territory (Probatova, 2014, and references therein); 2n = 20 was defined for the Primorye Territory (Probatova, 2014, and references therein).
Tetraploid (4x), x = 10. Distribution: Eurasian species, common in all regions of Siberia. At the beginning of the 20th century the species was known in most of the Tomsk province (which included the Novosibirsk Region) in the fields, near houses and roads, sometimes in meadows (Krylov, 1901). To date, it has been registered in 18 districts of the region .
Distribution: native to Europe and North Africa. It is widely cultivated as an ornamental plant, including in Siberia, and often goes wild. In the Novosibirsk Region, it was noted to become naturalized in 2014 ; it is abundant in the discovered habitats .
Distribution: the European-Mediterranean species, which has spread throughout the globe. It is one of the active invasive species in Siberia (Ebel et al., 2014). Species was introduced to the Novosibirsk Region in the 1960s (Krylov, Sergievskaya, 1964), it is known in ten districts of the region .
The chromosome number is given for the Novosibirsk Region for the first time. The same chromosome number was counted from the Irkutsk Region (Chepinoga, 2014, and references therein), the Primorye Territory (Probatova, 2014, and references therein), the Khabarovsk Territory (Probatova et al., 2011), the Republic of Ingushetia (Efimov, 1987), and the Republic of Altai .
Distribution: the East-Asian species that has spread to Europe and North America. It belongs to the invasive plants in Central Russia (Vinogradova et al., 2010) and Siberia (Ebel et al., 2014). In the Novosibirsk Region, it is noted as common in all inhabited districts (Shaulo, 2000), however, specimens in herbarium are only from the Novosibirsk city .
The chromosome number is given for the Novosibirsk Region for the first time. The same number was determined for the Krasnoyarsk Territory (Stepanov, 1994), the Irkutsk Region (Chepinoga, 2014, and references therein), the Primorye Territory (Probatova, 2014, and references therein) and the Amur Region (Probatova et al., 2006). Diploid (2x), x = 8.
Distribution: the eurasian species, rare in the regions of Siberia. Since the 1930s it has been known in the Novosibirsk Region (Krylov, 1937), now it is rarely found in most areas of the region .
The chromosome number for the Novosibirsk Region is given for the first time. The same number was determined for the Krasnoyarsk Territory (Stepanov, Muratova, 1995) and the Irkutsk Region (Probatova et al., 2018).
Distribution: the native species to Europe, the Caucasus, Western and Central Asia, introduced to the Far East and North America. It is grown as a spice and medical plant in most regions of Western Siberia, occasionally becomes wild. It was first recorded in the Novosibirsk Region in 2010 from Novosibirsk city (Zykova, 2015); by now, it is extremely rare here .
The chromosome number was determined on the Siberian material for the first time. The number 2n = 34 and 2n = 36 was revealed for the Primorye Territory (Probatova, 2014, and references therein).
Distribution: Holarctic species. In Siberia, including the Novosibirsk Region, it is a widespread ruderal weed.
The chromosome number was determined on the Siberian material for the first time. The same number was determined for the Primorye Territory (Probatova, 2014, and references therein).
Distribution: North American species spreading throughout the Holarctic. In the European part of Russia, it is one of the most aggressive invasive species (Vinogradova et al., 2010). It spreads actively across Siberia (Ebel, 2013;Buko, 2016). In the Novosibirsk Region, it was found in the Ust-Tarksk district (Ebel, 2008) and in the city of Novosibirsk (Zykova, 2015).
The chromosome number is given from the Novosibirsk Region for the first time. The same number was determined for the Tomsk Region (Probatova et al., 2016).
Distribution: the South Asian species with a cosmopolitan secondary range that has settled in the regions of Southern Siberia. It is included in the list of invasive and potentially invasive species of Siberia (Ebel et al., 2014) and the Black Book of Siberian Flora (Sheremetova, 2016). The species has been noted since the early 20th century in the Novosibirsk Region (Krylov, 1928), now it is quite common here.
The chromosome number is given for the Novosibirsk Region for the first time. The same one was Zykova E. Yu., Pankova (An'kova) T. V. Chromosome numbers in some alien plants of Novosibirsk Region counted for the Irkutsk Region (Chepinoga, 2014, and references therein), the Amur Region (Probatova, Sokolovskaya, 1983), the Primorye Territory (Probatova, 2014, and references therein) and the Kamchatka Territory (Probatova et al., 2017). Tetraploid level (2n = 36) was determined for the Krasnoyarsk Territory (Stepanov, Muratova, 1992).

POLYGONACEAE
Distribution: the far-eastern species grows on Sakhalin, the Kuril Islands, Japan, China. Species was growing as an ornamental plant, remains in old fallow expositions of the Central Siberian Botanical Garden, actively spreads in ravines and forests, forming extensive thickets in glades .
The chromosome number on the Siberian material was determined for the first time. The same number was determined from the Sakhalin Region (Probatova et al., 2007).

Conclusion
The chromosome numbers of 12 alien species from West Siberian populations (except for Brassica rapa subsp. oleifera) were studied for the first time. Seven species are diploids with high basic numbers (x = 7, 8, 10, 11, 17), five are polyploids (tetraploids Senecio viscosus, Epilobium pseudorubescens, hexaploids Campanula rapunculoides, Malva pusilla and Echinochloa crus-galli). Our data on ploidy level of the species from West Siberian populations is compared with those literature data on the populations of Eastern Siberia and the Far East.