Chromosome numbers in some alien plant species of the Novosibirsk Region: post IV

Summary . This paper presents the chromosome numbers (2 n ) for 12 alien species collected in the Novosibirsk Region from the families Apocynaceae, Asteraceae, Campanulaceae, Caryophyllaceae, Fabaceae, Lamiaceae, Plantag-inaceae, Poaceae. For Asclepias syriaca L. (2 n = 22), Veronica filiformis Sm. (2 n = 14), Setaria italica (L.) P. Beauv. (2 n = 18), and Sorghum drummondii (Nees ex Steud.) Millsp. et Chase (2 n = 20), chromosome numbers were determined for the first time on the material from Russia; for Tanacetum parthenium (L.) Sch. Bip. (2 n = 18), Campanula persicifolia L. (2 n = 16), and Genista tinctoria L. (2 n = 48) – from Asian Russia; for Calendula officinalis L. (2 n = 32) and Rudbeckia hirta L. (2 n = 38) – from Siberia. Chromosome numbers on the material from the Novosibirsk Region were revealed for the first time for Lapsana communis L. (2 n = 14) and Silene noctiflora L. (2 n = 24). For all the species studied, brief notes on their general distribution and dispersal in the Novosibirsk Region are provided, along with literature data on chromosome numbers from other regions of Russia.

Chromosome numbers were counted by direct observation in metaphase in root meristem squash preparations according to the method used by Zykova and Pankova (2021).Metaphase plates were observed under 100× magnification of the Axioscope 40 (Karl Zeiss, Axio Lab) microscope and photographed with an AxioCam MRc 5 digital camera.For all species, literature references on chromosome numbers known on material collected in Russia are given.Latin names of plants are provided according to the "Catalog of Life" (Hassler, 2023).Voucher specimens have been deposited to the Herbarium of the Central Siberian Botanical Garden SB RAS (NS, Novosibirsk).
Distribution: The native area of Asclepias syriaca covers North America and adjacent areas of Canada.It was previously experimentally cultivated as a rubbery species and now is used as ornamental and melliferous plant.The species has widely settled in the countries of Central and Southern Europe, where it is prohibited for cultivation (Asclepias syriaca, 2023).In Siberia, it was first found outside of culture in the Altai Territory (Terekhina, 1998).Recently discovered in the Novosibirsk Region and the Republic of Altai (Ebel, Zykova, 2022).
The chromosome number was determined for the first time in Russia.The same chromosome number was reported for some countries of Eastern Europe (Rice et al., 2015).
Distribution: Mediterranean species widely cultivated all around the world.In the Novosibirsk Region, it was recorded in the Novosibirsk agglomeration (Zykova, Shaulo, 2020, 2021;Zykova et al., 2022b).However, as the species easily spreads from cultivated sites, it can be found in other districts of the region.This is the first report of the chromosome number for Siberia.The same number is determined for the Primorye Territory (Probatova, 2014, and references therein).
Distribution: Euro-Mediterranean species with a Holarctic secondary range.Known in most regions of Southern Siberia (Ebel et al., 2014).It was first discovered in the Novosibirsk Region in 2017 in Akademgorodok of the Novosibirsk City (Shaulo, Zykova, 2018), where it still remains in these habitats.This is the first report of the chromosome number for the Novosibirsk Region.The same chromosome complement has been published earlier from the Republic of Karelia and the Leningrad Region (Probatova et al., 2009), the Republic of North Ossetia-Alania (Probatova, Seledets, 2008), the Krasnodar Territory (Probatova et al., 2013), and from the Republic of Altai (Zykova et al., 2020).
Distribution: North American species, widely cultivated as an ornamental plant, sometimes runs wild.In the Novosibirsk Region, outside of culture, it was first noted in the village Payvino (Krylov, Sergievskaya, 1964).To date, it is mentioned for the Novosibirsk City and surroundings (Zykova, 2019;Zykova, Shaulo, 2020;Zykova et al., 2022b).Chromosome numbers in some alien plant species of Novosibirsk Region: post IV This is the first report of the chromosome number for Siberia.The same number is determined for the Primorye Territory (Probatova, 2014, and references therein).
Distribution: European-Mediterranean species cultivated as ornamental plant, running wild.Outside of culture it was registered in Akademgorodok of the Novosibirsk City (Shaulo, Zykova, 2013, 2022).This is the first report of the chromosome number for Asian Russia.The same chromosome number was determined in material from the Primorye Territory (Probatova, 2014, and references therein).
Distribution: European species, occasionally cultivated.In the Novosibirsk Region, it was discovered as a wild in 2018; by now, it is known in the Novosibirsk City and its surroundings (Zykova, Shaulo, 2019, 2021).This is the first report of the chromosome number for Asian Russia.The same chromosome number was mentioned for the Leningrad Region (Agapova et al., 1990).
Distribution: European species, distributed in the Northern Hemisphere.It is included in the list of invasive and potentially invasive species of Siberia (Ebel et al., 2014).In the Novosibirsk Region, only two localities of the species are known: on the territory of the Central Siberian Botanical Garden in the Novosibirsk City (Zykova et al., 2014) and in the village of Lozhok in the Novosibirsk District (Shaulo, Zykova, 2022).This is the first report of the chromosome number for the Novosibirsk Region.The same number was recorded from the Republic of Altai (Lomonosova et al., 2018), the Sakhalin Region (Probatova et al., 2007, andreferences therein), the Krasnodar (Magulaev, 1992) and the Altai (An'kova et al., 2019) Territories.The number 2n = 36 was registered in material from the Altai Territory (An'kova et al., 2019).
Distribution: European species, often cultivated, rarely runs wild.In the Novosibirsk Region, outside of cultivation, it has been recorded in the Novosibirsk City (Akademgorodok) (Shaulo, 2000).To date, it has been registered in the Novosibirsk City and its surroundings (Zykova, 2019).This is the first report of the chromosome number for Asian Russia.The same chromosome number was mentioned for the Stavropol Territory (Magulaev, 1980).
Distribution: European-Caucasian-Central Asian species, penetrated in Siberia and the Far East.On the territory of the Novosibirsk Region, it was already known by the beginning of the 20th century (Krylov, 1907).Now it is pointed out for 9 districts of the region (Zykova, 2019).
The same number was recorded from the Irkutsk Region (Chepinoga, 2014, and references therein), the Primorye (Probatova, 2014, and references therein) and the Khabarovsk (Probatova et al., 1991) Territories.The number 2n = 20 was registered in material from the Novosibirsk Region (Krasnikov, 1991).
Diploid (2x), x = 7. Distribution: Caucasian-West Asian species with a secondary Holarctic range.In Siberia, it is grown as a ground cover and easily runs wild.In the Novosibirsk Region, it was first discovered in 1998 in the valley of the river Zyryanka (Shaulo, Durnikin, 2001).By now, it has spread widely across Akademgorodok, where is abundant and gives a background when flowering (Zykova, 2019).
The chromosome number was determined for the first time in Russia.The same chromosome number was reported for Europe (Rice et al., 1915).
Distribution: Cultivated plant predominantly in subtropical regions of the Earth with an unknown primary range, rare in Siberia.It was found in the Novosibirsk Region in 2020 (Shaulo, Zykova, 2022).
The chromosome number was determined for the first time in Russia.The same chromosome number was reported for different countries of Eurasia (Rice et al., 1915).
Distribution: An African species widely cultivated as a fodder plant, as adventive species is very rare in Siberia, found in the Novosibirsk Region in 2020 (Shaulo, Zykova, 2022).
The chromosome number was determined for the first time in Russia.The same chromosome number was reported for Europe, Near East and India (Rice et al., 2015), most counts were made on cultural material.

Conclusion
The study provides the first records of chromosome numbers for 12 alien species based on the material from the Novosibirsk Region.Most species are diploids.Three species are tetraploids: Calendula officinalis, Genista tinctoria, and Sorghum drummondii.Our data correlate with data on chromosome numbers determined on the material from the regions of East Siberia, the Far East, and European Russia.